摘要
背景:由于骨髓源性间充质干细胞的提取造成患者的二次损伤,也存在病毒、细菌污染的可能,扩增、分化能力不高,为此,实验从胎盘中提取间充质干细胞进行研究,了解其分泌抗凋亡细胞因子的情况,探讨其代替骨髓间充质干细胞的可能性。目的:体外分离培养、扩增人胎盘源性间充质干细胞,研究其基本生物学特性和抗凋亡细胞因子的分泌水平。方法:提取人胎盘间充质干细胞,显微镜下观察细胞形态;采用流式细胞仪测定细胞周期、凋亡情况及表面标记的表达,研究其增殖和生长特性。ELISA检测血管内皮细胞生长因子、胰岛素样生长因子1、肝细胞生长因子的分泌水平。结果与结论:在体外培养条件下,人胎盘间充质干细胞为贴壁生长,为成纤维细胞样,核浆比大,细胞增殖活跃,凋亡率低,可分泌能抑制细胞凋亡的细胞因子如血管内皮细胞生长因子、胰岛素样生长因子1、肝细胞生长因子。
BACKGROUND：The secondary injury to patients by extraction of bone marrow mesenchymal stem cells also has the possibility of virus and bacterial contamination,and the ability of amplification and differentiation was not high.Therefore,this study extracted mesenchymal stem cells from placenta to understand the condition of secreting anti-apoptosis cytokine,and to explore the possibility of replacing bone marrow mesenchymal stem cells.OBJECTIVE：To isolate,culture in vitro,amplify human placenta derived mesenchymal stem cells,and to study the biological character and secretion levels of placenta derived mesenchymal stem cells.METHODS：After isolating mesenchymal stem cells from the placenta,cell morphology was observed under microscope.Cell cycle,apoptotic cell death and the surface labeling expression were evaluated using flow cytometry to study their proliferation and growth characteristics.The cytokines,including vascular endothelial growth factor（VEGF）,hepatocyte growth factor（HGF） and insulin-like growth factor-1（IGF-1）,were detected using enzyme-linked immunosorbent assay.RESULTS AND CONCLUSION：Human placenta mesenchymal stem cells were fibroblast-like,with increased nuclear cytoplasm ratio under in vitro culture.The proliferation ratio was increased,while apoptosis decreased.Human placenta mesenchymal stem cells secreted anti-apoptotic cytokines,such as VEGF,IGF-1,and HGF.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第6期1005-1008,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
