摘要
目的研究阿尔茨海默病(AD)模型大鼠海马中神经元核抗原(NeuN)、白细胞介素1(IL-1)的表达及丁苯酞对其影响。方法成年雄性SD大鼠60只,随机分为模型组、丁苯酞组及对照组,每组20只,采用立体定位仪向左侧海马微量注射β淀粉样蛋白1-42(Aβ1-42)的方法建立AD大鼠模型,通过Y-形电迷宫检测造模前及造模后60天大鼠的学习记忆能力;喂养60天以后取各组大鼠脑组织,进行NeuN和IL-1免疫组织化学染色。结果与对照组相比,模型组大鼠学习记忆能力下降,其海马各区NeuN阳性细胞明显减少,IL-1表达增多,差异有统计学意义(P<0.01);与模型组相比,丁苯酞组大鼠学习记忆能力有所改善,其海马各区NeuN阳性细胞明显增多,IL-1表达减少,差异有统计学意义(P<0.05)。结论丁苯酞能够改善AD大鼠的学习记忆能力,可能与抑制IL-1的表达、减少对神经细胞的损伤有关。
Objective To determine the expression of neuronal nuclei（NeuN） in the CA1 region of hippocampus and Interleukin - 1 （IL -1 ） in the junction of cortex and CA1 region of hippocampus in model rats with Alzheimer disease （AD） , and to determine the effect of butylphthalide on them and its significance. Methods Sixty male adult rats were randomly divided into a model group, a butylphthalide group, and a control group. AD models were established by injecting beta -amyloid protein 1 -42 （Aβ1 -42） into the hippocampus of rats. The learning and memory ability were evaluated by Y - type electric maze before and sixty days later. The rats were sacrificed and both sides of the hippocampus were sectioned for immunohistochemistry. Results The learning and memory abilities reduced, positive cells of NeuN in the hippocampus of the model group decreased and the expression of IL - 1 increased statistically,compared with the control group（P 〈0.01 ）. The learning and memory abilities improved, the positive cells of NeuN in the hippocampus of the butylphthalide group increased and the expression of IL- 1 decreased statistically,compared with the model group（P 〈 0.05 ）. Conclusion Butylphthalide has an effect of improving learning and memory ability of AD model rats, which is possibly due to the reduce of the expressions of IL - 1 and the proteetion of the nerve tissue.
出处
《医学研究杂志》
2011年第3期67-70,共4页
Journal of Medical Research
基金
湖南省自然科学基金资助项目(09JJ3058)
湖南省科学技术厅科技计划项目(2009SK3175)
