摘要
目的探讨PTEN异常表达及其基因启动子区异常甲基化与甲状腺乳头状癌发生、发展及转移的关系。方法采用免疫组化、western-blot、甲基化特异性PCR(MSP)分析正常甲状腺与甲状腺乳头状癌组织中PTEN蛋白表达差异及PTEN基因启动子区甲基化情况。结果与正常甲状腺组织相比,甲状腺乳头状癌组织中PTEN蛋白表达明显下调(P<0.01),同时笔者发现PTEN基因蛋白的下调与肿瘤的淋巴结转移状况呈负相关(P<0.005),且甲状腺乳头状癌组织中PTEN基因启动子区甲基化程度显著增高。结论 PTEN蛋白低表达是甲状腺乳头状癌的发生、转移的重要原因之一,其低表达与基因启动子区过度甲基化有关。
Objective To investigate the protein expression and promoter Methylation of PTEN gene in papillary thyroid carcinoma and normal thyroid tissue and its biological significants. Methods Methylation -specific PCR,immunohistochemical and western -blot were used to analyze the promoter Methylation and protein expression differences of PTEN gene in tissues of papillary thyroid carcinoma and normal thyroid. Results Compared with normal thyroid tissue, the PTEN protein was significantly reduced （P 〈 0.01） in papillary thyroid cancer,while the reduction of PTEN protein was negatively correlated with tumor stage （P 〈 0. 005）and promoter methylation of PTEN was more common. Conclusion Promoter hypermethylation of PTEN in papillary thyroid carcinoma causes decreased expression of PTEN,which may be one of major reason for the metastasis in papillary thyroid carcinoma.
出处
《医学研究杂志》
2011年第3期39-43,共5页
Journal of Medical Research
基金
国家"863"计划重点项目(2006AA02090406B)
