摘要
老挝纹胸鮡是分布于澜沧江流域的一种特有鱼类,喜激流底栖生活。为了解其各地理种群间的遗传结构与遗传变异,文章分析了129尾采自澜沧江8条支流水系的老挝纹胸鮡mtDNA细胞色素b基因1 138 bp的序列变异,共发现16个多态性位点,定义了15个单倍型。澜沧江老挝纹胸鮡遗传变异较低,平均单倍型多样性指数和核苷酸多样性指数分别是h=0.299,π=0.299,有3个种群甚至没有变异,分子变异方差分析结果表明各种群内变异大于各种群间变异,种群间没有差异。对老挝纹胸鮡所有种群进行的单倍型错配分布呈现单峰型,中性检验结果均为负值(Tajima's D=?2.36965,P<0.02;Fu's Fs=?20.975,P<0.05)表明,澜沧江老挝纹胸鮡自然种群可能经历过近期的种群扩张事件。
The hillstream catfish,Glyptothorax laosensis,is endemic to basin of Lancang River(also known as Mekong River).Genetic variation was detected within and among 8 populations of G.laosensis in Lancang River based on mito-chondrial cytochrome b sequences.A total 16 nucleotides sites were detected in 1 138 bp fragment,which defined 15 hap-lotypes from 129 sequences.The variation level was very low,with mean h=0.299 and π=0.00032,respectively,and no variation was detected in some populations.Hierarchical analysis of molecular variance(AMOVA) showed no significant genetic structuring among populations.The unimodel mismatch distribution of pairwise haplotype difference and the nega-tively selective neutrality test(Tajima's D=?2.3696,P0.02;Fu's Fs=?20.975,P0.05) suggested that a recent population expansion had occurred.
出处
《遗传》
CAS
CSCD
北大核心
2011年第3期255-261,共7页
Hereditas(Beijing)
基金
国家环保部项目资助