摘要
目的:研究异丙酚对第三丁基过氧化氢(t-BHP)诱导的脐静脉内皮细胞(HUVECs)氧化应激的保护作用和机制。方法:体外培养的HUVECs分为对照组、异丙酚组、t-BHP组、异丙酚预处理+t-BHP组,给予相应处理后,Westernblot检测p38MAPK磷酸化水平变化,RT-PCR检测iNOS、eNOS表达。结果:t-BHP处理后,能显著诱导p38MAPK磷酸化,激活iNOS、eNOS表达,而异丙酚预处理后能减轻这些变化。结论:异丙酚通过抑制p38MAPK,减少iNOS、eNOS表达,减轻氧化应激从而起到保护HUVECs的作用。
Objective To investigate the protective effect and mechanism of propofol on human umbilical vein endothelial cells （HUVECs） following oxidative stress induced by ten-butyl hydroperoxide （t-BHP）. Methods Cultured HUVECs were divided into four groups： control group, t-BHP group, propofol group, and pre-ineubated with propofol and treated with t-BHP group. Levels of p38 MAPK phosphorylation were measured by Western blot, and expressions of iNOS and eNOS were measured by RT-PCR. Results The phosphorylation of p38 MAPK was significantly induced, and the expressions of iNOS and eNOS were significantly increased while treated with t-BHP, which could be attenuated by propofol. Conclusions By inhibiting p38MAPK and decreasing iNOS and eNOS expression, propofol could protect HUVECs from oxidative stress.
出处
《实用医学杂志》
CAS
北大核心
2011年第9期1523-1525,共3页
The Journal of Practical Medicine
基金
国家自然科学基金资助项目(编号:30872431)
