摘要
背景:采用传统水提法提取的消淤接骨散药效不明显,推测可能为有效成分提取不充分所致。目的:正交实验进行消淤接骨散醇提取工艺设计。方法:以处方的醇浸膏得率和君药骨碎补有效成分柚皮苷含量为考察指标,采用L9(34)正交实验法进行优选,HPLC法测定柚皮苷含量,对消淤接骨散醇提取工艺进行优化筛选。观察醇浸膏得率,柚皮苷含量及吸醇率。结果与结论:第3次醇提的醇浸膏得率及柚皮苷提取量所占比率均大于10%,应进行3次醇提方可完全提取药物成分。药材吸醇率平均值为265.67mL/g,表明提取时第1次应多加3倍量溶剂。正交实验结果分析,由极差R值大小显示,各因素作用主次为加醇量>乙醇体积分数>提取时间,方差分析结果表明:乙醇体积分数、加醇量两因素的影响具有显著性意义(P<0.05)。最佳优选工艺即药材加乙醇回流提取3次,第1次加6倍量体积分数65%乙醇提取2h,第2次加5倍量体积分数65%醇提取1.5h,第3次加5倍量体积分数65%醇提取1.5h。较以往中药提取工艺可更有效得出药物有效成分、节省不必要的工艺流程,且该工艺操作简单,重复性好,柚皮苷含量高。
BACKGROUND：The drug effect does not show apparently through the method of aqueous extraction for the connecting bones and reducing swelling powder.The reason could be that the effective components cannot be extracted sufficiently.OBJECTIVE：To optimize the ethanol extraction technology from the powder of connecting bones and reducing swelling by orthogonal trial.METHODS：The ethanol extraction rate in prescription and Naringin volume,which is an effective component of principal drug rhizoma drynariae,were taken as the measurement index,for the optimization through L9（34） orthogonal trial.Naringin content was determined by high-performance liquid chromatography method and the ethanol extraction techniques from connecting bones and reducing swelling powder were optimized.The ethanol extraction rate,Naringin volume,and the rate of alcohol absorption were observed RESULTS AND CONCLUSION：The percentages of ethanol extraction rate and Naringin volume were more than 10% in the third ethanol extraction.The components of Chinese herbal medicine can be completely extracted through three ethanol extractions.The average of alcohol absorption rate was 265.67 mL/g,which means more than 3 times of menstruum would be added in the first ethanol extraction.The result of orthogonal test showed that the dosage of menstruum was the key factor,the ethanol volume fraction was the subordination factor and the time cost in extraction was the most subordination factor,assayed by range R value.The analysis of variance showed that there were significant differences in the effect of the ethanol volume fraction and the dosage of menstruum（P 0.05）,so the optimized extraction condition was：65% ethanol refluxed and extracted for three times,six times of ethanol extracted for 2 hours,five times for 1.5 hours,five times for 1.5 hours respectively.The optimized extraction technique is superior to routine technology regarding extracting drug components,thus avoiding unwanted technology process.The optimized process is repeatable,simply operated and could reach high naringin content.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第8期1433-1436,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
广东省科技计划项目(2006B35802005),课题名称:"纳米中药功能衬材的实验和应用研究"~~
