摘要
背景:纳米级的羟基磷灰石纤维蛋白凝胶材料与人体内组织成分更为相似,具有良好的生物与力学性能,但缺乏骨诱导作用。目的:观察纳米羟基磷灰石纤维蛋白凝胶/重组人成骨蛋白1复合人工骨的骨缺损修复能力。方法:制备新西兰大白兔单侧桡骨缺损模型后,以数字表法随机分为3组,分别植入不同材料行骨缺损修复:纳米羟基磷灰石纤维蛋白凝胶/重组人成骨蛋白1人工骨组、纳米羟基磷灰石/纤维蛋白凝胶组、空白对照组(未植入任何材料)。术后4,8,12周行大体标本观察、X射线、扫描电镜、放射性核素骨扫描及生物力学测试,比较各组材料修复骨缺损的能力。结果与结论:术后4,8,12周,纳米羟基磷灰石纤维蛋白凝胶/重组人成骨蛋白1人工骨组X射线评分、成骨效果、放射性核素聚集强度、生物力学强度均高于纳米羟基磷灰石/纤维蛋白凝胶组(P < 0.05)。空白对照组骨缺损区无骨性连接,骨端硬化,骨缺损未能修复。说明纳米羟基磷灰石纤维蛋白凝胶/重组人成骨蛋白1复合人工骨具有良好的骨缺损修复能力,有望成为一种理想的骨缺损修复材料。
BACKGROUND：The nano-hydroxyapatite/fibrin glue（Nano-HA/FG） is similar to the human tissues and possesses good biological and mechanical properties,but it lacks of bone induction effect.OBJECTIVE：To investigate the repair capacity of nano-hydroxyapatite/fibrin glue/recombinant human osteogenic protein-1（Nano-HA/FG/rhOP-1） on bone defects.METHODS：Animal models of bone defects were established on the bilateral radius of New Zealand white rabbits,which were randomly divided into Nano-HA/FG/rhOP-1 artificial bone group（bone defect was repaired with artificial bone）,Nano-HA/FG artificial bone group（bone defect was repaired with Nano-HA/FG artificial bone） and blank control group（bone defect was left unrepaired）.The ability of repairing bone defect was evaluated by gross specimen observation,X-ray radiograph,scanning electronic microscope,radionuclide bone scan,and biomechanical test at 4,8,12 weeks after operation.RESULTS AND CONCLUSION：Radiographical scores,osteogenic effect,aggregation intensity of radionuclide,and biomechanical strength in Nano-HA/FG/rhOP-1 artificial bone group were higher than that in Nano-HA/FG group（P 0.05） at 4,8,12 weeks after operation.There was no bone connection in the defect field,bone end sclerosis,and the bone defect was not repaired in the blank control group.It suggested that Nano-HA/FG/rhOP-1 artificial bone has good repair capacity of bone defects and is expected to be an ideal repairing material for bone defect.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第8期1360-1364,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
深圳市科技计划项目资助~~
