摘要
研究天府肉鸭腔上囊胚胎及胚后发育期Bcl-2、Bax、Fas、FasL蛋白的表达。将20只天府肉鸭分为4组,即24天胚龄(E24),胚后3、8、29周龄(P3、P8、P29),采用免疫组化技术。结果显示,Bcl-2、Bax、Fas、FasL在各组滤泡淋巴细胞中均有表达,FasL还表达于滤泡间上皮。滤泡皮质和髓质淋巴细胞Bcl-2阳性率呈递减的变化趋势(髓质P3～8除外);皮质和髓质淋巴细胞Bax阳性率在E24～P8恒定,P29上升;皮质和髓质淋巴细胞Bcl-2/Bax值呈下降趋势,但皮质在P3～8恒定,髓质在P3～8上升;滤泡皮质和髓质淋巴细胞Fas阳性率在E24～P3上升,P3～8下降,P8～29上升;皮质和髓质淋巴细胞FasL阳性率变化规律与Fas相似,但皮质在P3～8恒定。滤泡皮质淋巴细胞Bcl-2阳性率及Bcl-2/Bax值在E24～P8明显高于髓质,在P29则显著低于髓质;皮质淋巴细胞Bax阳性率在E24～P8与髓质无显著差异,在P29则明显高于髓质;皮质淋巴细胞Fas和FasL阳性率在E24～P8明显低于髓质,在P29则显著高于髓质。结果提示,Bcl-2、Bax、Fas、FasL是鸭腔上囊胚胎及胚后发育期淋巴细胞凋亡的重要调节物。
This experiment was conducted to study the expression of Bcl-2,Bax,Fas and FasL proteins during the embryonic and post embryonic development of the Tianfu duck bursa of Fabricius.20 Tianfu ducks were divided into 4 groups as follows： on embryonic day 24（E24）,and post-embryonic week 3,8 and 29（P3,P8,P29）.Immunohistochemisty technique was used in this study.The results showed that Bcl-2,Bax,Fas and FasL were expressed in the follicle lymphocytes,and FasL was also displayed in the interfollicular epithelia.The Bcl-2 positive ratios of lymphocytes in the follicle cortex and medulla progressively decreased（except P3-8 in the medulla）.The Bax positive ratios of lymphocytes remained stable from E24-P8,but dramatically rose on P29.The values of Bcl-2/Bax of lymphocytes showed a decreased tendency;however,they remained unchanged in the cortex and rose in the medulla from P3-8,respectively.The Fas positive ratios of lymphocytes increased from E24-P3 and P8-29,but decreased from P3-8.The changing pattern of FasL positive ratios of lymphocytes was similar to that of Fas,however,it remained stable from P3-8.Furthermore,the Bcl-2 positive ratios and the values of Bcl-2/Bax of lymphocytes in the follicle cortex were significantly higher from E24-P8 and lower on P29 than those in the follicle medulla,respectively.The Bax positive ratios of lymphocytes in the follicle cortex were not significant different from those in the medulla from E24-P8,but higher than those in the medulla on P29.The Fas and FasL positive ratios of lymphocytes in the follicle cortex were significantly lower from E24-P8 and higher on P29 than those in the medulla.This study indicated that Bcl-2,Bax,Fas and FasL proteins were important modulators for the lymphocyte apoptosis of the duck bursa of Fabricius during the embryonic and post embryonic development.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2011年第3期416-422,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
教育部长江学者和创新团队发展计划(0848)
