摘要
【目的】初步探讨植物乳杆菌LpT1和LpT2的体外降胆固醇机制。【方法】以MRS、MRS+CH、MRS+CH+S和MRS+CH+N四种培养基为基础,接种植物乳杆菌LpT1和LpT2进行培养,通过分析比较培养基上清液、菌体沉淀和菌体细胞内部胆固醇含量以及接种和未接种两种情况上清液、沉淀和细胞内胆固醇总量变化,推测植物乳杆菌体外降胆固醇机制。【结果】乳酸菌体外降胆固醇存在非代谢和代谢降解两条途径,非代谢途径与共沉淀作用和菌体吸收有关。代谢降解是由于植物乳杆菌在生长过程中产生了特殊的酶系,从而将胆固醇降解成其他物质,导致其含量降低。【结论】研究结果为进一步研究植物乳杆菌体外降胆固醇的机制奠定了良好基础。
[Objective]To explore the mechanism of cholesterol-degrading by Lactobacillus plantarum LpT1 and LpT2 in vitro.[Methods]After Lactobacillus plantarum LpT1 and LpT2 being inoculated and cultured in the medium MRS,MRS ＋ CH,MRS ＋ CH ＋ S and MRS ＋ CH ＋ N,the cholesterol content in supernatant,deposit and cells,and the total cholesterol content before and after inoculation were determined and compared to primarily predict the mechanism of cholesterol-degrading by L.plantarum.[Results]The mechanism of cholesterol-degrading by L.plantarum LpT1 and LpT2 in vitro involved in metabolic and non-metabolic pathway.Non-metabolic pathway was related to co-precipitation and cell absorption.Metabolic pathway was due to the production of the special enzymes during the growth of L.plantarum which made the cholesterol degrade to be other materials and caused its content reduction.[Conclusion]L.plantarum has shown its ability to degrade cholesterol in vitro.
出处
《微生物学报》
CAS
CSCD
北大核心
2011年第4期561-565,共5页
Acta Microbiologica Sinica
基金
"十一五"国家科技支撑计划重点项目--"功能性食品的研制和开发"之"食品功能因子高效分离与制备关键技术的研究"(2006BAD27B03)~~
关键词
植物乳杆菌
降胆固醇
机制
Lactobacillus plantarum
cholesterol-degrading
mechanism
