摘要
目的探讨非小细胞肺癌(NSCLC)患者中紧密连接蛋白-1(ZO-1)基因甲基化检测的临床意义。方法应用甲基化特异性聚合酶链反应MS-PCR检测101例NSCLC患者癌组织及癌旁组织ZO-1基因启动子甲基化状态,以61例肺部良性病变患者作为对照,并用实时荧光定量PCR和Western blotting分别检测63例甲基化阳性组和200例阴性组中的mRNA和蛋白表达。结果 ZO-1 mRNA和蛋白在启动子甲基化阳性和阴性组中的差异有显著统计学意义(t=-26.028,P<0.01;t=-37.216,P<0.01)。ZO-1启动子甲基化在癌组织(32.7%,33/101)和正常组织中(11.5%,7/61)的差异有显著统计学意义(χ2=9.190,P<0.01)。癌旁组织ZO-1启动子甲基化在淋巴结转移组(35.5%,11/31)和未转移组中(17.1%,12/70)中的差异有显著统计学意义(χ2=4.110,P<0.05),癌组织ZO-1启动子甲基化在2年生存组(27.5%,14/51)和2年死亡组(43.2%,19/44)中的显著差异有统计学意义(χ2=4.150,P<0.05)。结论 ZO-1启动子甲基化影响了其mRNA转录和蛋白的表达,癌旁组织和癌组织ZO-1启动子甲基化分别可能作为NSCLC患者淋巴结转移和2年内生存的预测因子。
Objective The objective of this study is to investigate the methylation status of zonula occluden-1 （ZO-1） gene in patients with non-small cell lung cancer （NSCLC） and to indentify this roles in pathogenesis, development and classification of NSCLC. Methods The methylation status of ZO-1 gene of 101 patients with NSCLC and 61 patients with benign lung disease were detected by methylationspecific-polymerase chain reaction （MS-PCR）. ZO-1 mRNA and protein of 200 unmethylation tissue and 62 methylation tissue were detected by real-time PCR and Western blotting separately. Result Both of ZO-1 mRNA and protein was significant statistic different between methylation group and unmethylation group（ t = - 26. 028,P 〈 0.01, t = - 37. 216, P 〈 0.01 ）. There was significant statistic difference between carcinoma tissue group （32.7% ,33/100） and control group（ 11.5% ,7/61 ） （χ2 = 9. 190, P 〈 0. 01 ）. ZO-1 promoter methylation status in adjacent tissues was significant statistic different between lymph node invasion group（35.5% , 11/31 ） and lymph nodes without invasion group（ 17.1% , 12/70） （χ2 = 4. 110, P 〈 0.05 ）. ZO-1 promoter methylation status of carcinoma tissues wase significant statistic different between 2-year suvival group （27.5% ,14/51 ） and 2-year death group （43.2% ,19/44） （χ2 = 4. 150, P 〈 0. 05）. Conclusion Methylation status of ZO-1 promoter may affect the transcription of mRNA and expression of protein. ZO-1 promoter methylation status of adjacent tissues and carcinoma tissues in NSCLC patients may be supposed to forecast the lymph node invasion and 2-year survival rate separately.
出处
《解剖学报》
CAS
CSCD
北大核心
2011年第2期195-200,共6页
Acta Anatomica Sinica
基金
舟山市卫生局医药科研计划项目(2008B005)
舟山市科技局科技计划项目(91042)
