摘要
目的探讨姜黄素对人鼻咽癌CNE-2Z细胞增殖及凋亡的影响及其相关的分子机制。方法体外培养的CNE-2Z细胞经不同剂量姜黄素处理后,用MTT法观察对细胞增殖的影响,流式细胞术检测细胞周期和凋亡率的改变,Western blot检测姜黄素处理后相关蛋白Bcl-2、Bax表达水平的变化。结果姜黄素对CNE-2Z细胞的生长和增殖都具有一定程度的抑制作用,阻断细胞于S期和G2/M期。姜黄素处理后CNE-2Z细胞凋亡抑制基因Bcl-2蛋白表达减少,同时促凋亡基因Bax蛋白表达增加。结论姜黄素可抑制CNE-2Z细胞增殖并促进其凋亡。
Objective To study the effects of Curcumin on proliferation and apoptosis of human nasopharyngeal carcinoma cell CNE-2Z and its possible mechanism.Methods After treatment with Curcumin at various concentrations,the proliferative inhibition of CNE-2Z was observed by MTT assay.The cell cycle influence and cell apoptosis rate were detected by flow cytometry.The expression of Bcl-2 and Bax were detected by Western blot.Results Curcumin could inhibit the proliferation of human nasopharyngeal carcinoma cells CNE-2Z at a time and dose dependent pattern.The cells were blocked at G2/M and S phases.When treated with Curcumin for 24h,the percentage of cell apoptosis was increased.Bcl-2 was down-regulated whereas Bax was up-regulated.Conclusion Curcumin could inhibit the proliferation of CNE-2Z and induce cell apoptosis.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2011年第3期254-256,共3页
Cancer Research on Prevention and Treatment
基金
广东省教育厅自然科学研究基金资助项目(GK0404)
关键词
姜黄素
鼻咽癌
增殖
凋亡
BCL-2
BAX
Curcumin
Nasopharyngeal carcinoma
Proliferation
Apoptosis
Bcl-2
Bax
