摘要
了解食用菌产木质素氧化酶系的特征及其与菌丝生长速度的关系,采用RB-PDA脱色法、苯胺蓝平板脱色法等4种方法,检测32种食用菌中漆酶(Lac)、木质素过氧化物酶(Lip)和锰过氧化物酶(MnP),并且利用PDA平板实验测量其菌丝生长速度。结果表明:12个品种可同时分泌漆酶(Lac)、木质素过氧化物酶(Lip)和锰过氧化物酶(MnP),2个品种未检测到Lac、Lip、MnP,6个品种只分泌Lac,2个品种只分泌MnP,而未曾发现只分泌Lip的品种。说明食用菌对Lac和MnP的产生分泌具有独立性,但是木质素过氧化物酶必需与锰过氧化物酶同时分泌。木质素氧化酶系丰富食用菌菌种,菌丝生长速度快;Lac活性高的菌种,其生物转化率也高。
In order to understand relationship between the characteristics of the ligninolytic enzyme system producd by edible fungi and the the speed of hypha growth,the Lac,Lip and MnP of 32 species of edible funguses were analyzed by four methods including RB-PDA and Aniline blue decolonization in plate and so on.And the speed of hypha growth was measured by the plate of PDA.The result showed that 12 species had Lac,Lip and MnP,2 species had no enzymes,6 species only produced Lac,2 species only produced Mnp.However,there was not a specie which only produced Lip.All this shows that edible fungi have their own unique characteristics in secreting Lac and MnP,but Lip and MnP must be secreted at the same time.And the edible fungi which are rich in ligninolytic enzyme systeml grow faster then others,and the edible fungi with high Laccase activeness,their biological conversion rates are also high.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2011年第2期375-380,共6页
Acta Agriculturae Universitatis Jiangxiensis
基金
福建省科技重点项目(2009N0010)
关键词
食用菌
木素氧化酶系
生长速度
漆酶活力
edible fungus
ligninolytic enzyme system
growth velocity
laccase activity
