摘要
利用ＡＦＬＰ－银染法在一对花椰菜抗、感黑腐病的近等基因系中筛选到四个与抗黑腐病性状、一个与感黑腐病性状连锁的ＤＮＡ 分子标记．对其中一个４００ｂｐ 的抗病标记进行Ｓｏｕｔｈｅｒｎ 杂交检测，结果表明该标记在抗病品系中存在明显的杂交信号，而在感病品系中无杂交信号．该标记测序后，通过Ｇｅｎｂａｎｋ 进行同源性检测，发现其与拟南芥菜ＢＡＣ克隆Ｆ７Ｎ２２ 部分序列有７５％ 的同源性．该ＢＡＣ克隆位于拟南芥菜５ 号染色体的黑腐病抗性基因（ｒｘｃ２）附近．这意味着该标记可能与甘蓝黑腐病抗性基因紧密连锁．
Using AFLP silver staining method, we isolated five DNA markers from a pair of nearisogenic cauliflower lines, four of which are linked to the cabbage black rot ( Xanthomonas campestris pv. campestris )resistance, and one of which is linked to the susceptibility. Southern analysis of 400 bp marker linked to resistance showed that the hybridization signal is present in all resistant lines, but absent in the susceptible line. This marker has already been sequenced. The Genbank database searches reveales that it has 75% homology with a part of A. thaliana. BAC clone F7N22, which was located near the X. c. campestris resistance gene, rxc2 , on chromosome V of Arabidopsis thaliana. It demonstrated this sequence may be tightly linked to the cabbage black rot resistance gene.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第3期177-181,共5页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
天津市重点基金
