摘要
目的确定细粒棘球蚴抗原B亚单位3(EgAgB8/3)的特性并为研究其免疫功能奠定基础。方法根据已报道的编码EgAgB8/3的基因序列(AF362442)设计引物,进行基因扩增,利用DNAstarProtean软件对该抗原进行分析。结果成功克隆到EgAgB8/3基因,扩增片段为207 bp;序列比对结果显示,新疆细粒棘球蚴EgAgB8/3基因与GenBank登录号为AF362442序列完全一致,同源性为100%。对该抗原分析认为具有潜在的抗原表位位点。结论细粒棘球蚴EgAgB8/3在对包虫病的免疫诊断上是较好的候选抗原,并为研究该蛋白的免疫功能及建立以EgAgB8/3抗原为主的包虫病终末宿主免疫诊断方法奠定了基础。
Objective To further study the character of Echinococcus granulosus antigen B protein subunit B8/3（EgAgB8/3） gene and provide the basis for analysis of its immunological function.Methods The specific primers were designed according to published nucleotide sequence of Echinococcs granulosus EgAgB8/3 gene in the Genbank database（AF362442）.The fragment of EgAgB8/3 gene was amplified by PCR and analyzed by DNA star software.Results The EgAgB8/3 gene was successfully cloned and the full length of the cloned gene was 207 bp.The EgAgB8/3 gene showed 100% homology with the foreign strain（AF362442）.Several possible antigen epitopes located in the EgAgB8/3.Conclusion EgAgB8/3 has the potential immunological diagnosis value,it provides the basis for further establishing the immunological diagnosis method of Eg.
出处
《新疆医科大学学报》
CAS
2011年第3期236-239,共4页
Journal of Xinjiang Medical University
基金
国家自然科学基金项目(30760229)
科技部"863计划项目"(2007AA02Z411)
新疆维吾尔自治区高校科研计划(XJEDU2008S31)
