摘要
将重组鹿源BVDV基因E0植物表达载体(pBI121/E0),通过发根农杆菌Ri介导,用叶盘法转化得到人参发根,分别用PCR、RT-PCR检测其转化情况。结果表明:获得了转鹿源BVDV基因E0人参发根,鹿源BVDV基因E0已整合到人参发根基因组中并得到了转录,为研制转基因人参发根疫苗提供了科学依据和材料。
Ginseng hairy roots were induced by leaf disc method,and recombinant plant expression vector（pBI121/E0）of BVDV geneE0 of deer was transformed into ginseng hairy roots byAgobacterium rhizgenses with Ri-plasmid.E0 in ginseng hairy roots was detected by PCR,RT-PCR methods.The results indicated that E0 transgenic ginseng hairy roots were obtained.E0 was transferred into Ginseng hairy roots,and expressed in ginseng hairy roots.
出处
《中国农学通报》
CSCD
北大核心
2011年第10期239-242,共4页
Chinese Agricultural Science Bulletin
基金
"梅花鹿高效养殖加工关键技术的研究与应用"(2009GJB10031)
国家科技支撑计划项目"平地载参关键技术研究"(2007BAI38B01)
中国博士后科学基金项目"鹿源牛病毒性腹泻病毒E0基因在人参发根中表达及免疫原性研究"(20090461042)
