摘要
以马铃薯减数分裂二分体和四分体时期的小孢子为材料， 对原生质体分离的主要影响因素进行了研究。结果表明， １ ％ 蜗牛酶和０ ．５ ％ 崩溃酶的混合酶液游离效果最好， 原生质体的最高得率达６５．４ ％ 。渗透压调解剂以蔗糖最好， 甘露醇次之， 最适浓度分别为１６％和１８％ 。０ ．１ ％ 荧光增白剂检测表明脱壁完全， ＦＤＡ 荧光检测表明花粉原生质体具有生活力。
The pollen protoplast isolation from the microspores of Solanum tuberosum at dyad and tetrad stage of meiosis were studied.The result showed that 1% snailase and 0.5% driselase was the best enzyme combination for protoplast isolation,with a yield up to 65.4%.The best osmoticum is sucrose and the second is glucose and their optimum concentration was 16% and 18% respectively.Wall degraded pollens was identified with 0.1% calcoflower white test and its vitality was tested by fluorochromatic reaction with fluorescein diacetate(FDA).
出处
《园艺学报》
CAS
CSCD
北大核心
1999年第5期323-326,共4页
Acta Horticulturae Sinica
基金
国家教委优秀年轻教师基金
关键词
马铃薯
花粉原生质体
分离
Solanum tuberosum
Pollen protoplast
Isolation
