摘要
为快速评价短序与阔叶十大功劳不同部位化学成分差异,本研究运用FTIR采集短序和阔叶十大功劳不同部位的红外光谱并比较短序与阔叶十大功劳不同部位盐酸小檗碱含量差异。研究结果显示,生物碱、黄酮和丁香酯等化学成分,短序十大功劳根、茎及叶片中均较阔叶十大功劳丰富,但是,多糖和苷类含量在两种十大功劳根中相当,在茎和叶片中均以阔叶十大功劳含量高,尤其是叶片中这种差异更明显。最后,与盐酸小檗碱标准品比较发现,2种十大功劳中盐酸小檗碱含量均以茎中最高,叶片中含量最低;2种十大功劳比较各部位盐酸小檗碱含量均以短序十大功劳中高。故运用FTIR技术可以快速找出2种十大功劳化学成分的差异,本研究结果将为十大功劳属植物资源合理开发利用及良种选育提供参考。
In order to fast investigate the differences of chemical component between Mahonia breviracema （M.breviracema） and Mahonia healei （M.healei）,the fourier transform infrareds spectroscopy （FTIR） was used to detect the infrared spectroscopy spectral of different parts of M.breviracema and M.healei,respectively as well as compare their quantification differences of berbamine hydrochloride.The results showed that compared with M.healei,the content of some chemical compositions such as alkaloids,flavonoids,clove ester and so on were more abundant in different parts M.breviracema,but,when compare the content of polysaccharides and nucleoside,M.healei were more higher in stem and leaf than M.breviracema,furthermore,differences were more significant in leaf.Finally,compared with the standard of berbamine hydrochloride,the results demonstrate that berbamine hydrochloride content were had significant differences in different parts,and the content differences of berbamine hydrochloride were showed similiar regulations both in M.breviracema and M.healei,which the highest was stem,the lowest was leaf.When compare with the content of berbamine hydrochloride between M.breviracema and M.healei,the every parts of M.breviracema was higher than M.heale.Therefore,the chemical component differents between M.breviracema and M.healei were could be fast investigated when applied the technology of FTIR and the results of this research could provide reference for reasonable exploitation and breeding the resources of Mahonia.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2011年第2期224-228,共5页
Genomics and Applied Biology
基金
广西自然科学基金项目(2011GXNSFB018096)
桂林市科技成果转化与应用项目(20100103-6)
广西植物研究所科学研究基金项目(桂植业1009)共同资助
