摘要
目的观察还原型谷胱甘肽(GSH)预处理对肝脏缺血-再灌注损伤(HIRI)后糖代谢的影响。方法 78只SD大鼠随机均分为假手术组(C组)、缺血-再灌注组(IR组)和GSH预处理组(G组)。IR组和G组大鼠制作HIRI模型,C组仅开腹,不阻断肝门血管。每组分别在HIRI后2、6 h各取8只大鼠检测超氧化物歧化酶(SOD)、丙二醛(MDA)、血糖和胰岛素的含量,另取5只大鼠做高血糖钳夹试验后检测胰岛素含量,并计算葡萄糖代谢率(GMR)和胰岛素敏感指数(ISI)。结果与IR组比较,C组和G组血糖、MDA含量明显降低(P<0.05),胰岛素、SOD含量明显升高(P<0.05)。高血糖钳夹试验后,与IR组比较,C组和G组胰岛素含量、GMR及ISI均明显升高(P<0.05)。结论 GSH预处理可改善HIRI后的高血糖,其机制可能与GSH减轻HIRI过程中的氧化还原反应有关。
Objective To observe the effects of glutathione pretreatment on glucose metabolism after hepatic ischemia/reperfusion. Methods Seventy-eight SD rats were randomly divided into ischemia/reperfusion group （group IR）, glutathione group （group G）, and control group （group C）, with 26 rats in each group. The HIRI model was established in groups IR and G by ischemia for 40 min and reperfusion for 2 h or 6 h. The concentrations of blood glucose （BG） and plasma insulin, the activity of superoxide dismutase （SOD）, the levels of malondialdehyde （MDA）were determined at 2 h or 6 h after reperfusion in 8 rats of each group. Hyperglycemic clamp test was performed in another 5 rats of each group, so that to determine the concentration of insulin and to calculate the glucose metabolism rate（GMR） and the insulin sensitivity index（ISI）. Results Compared with those in group C, the concentrations of BG and the levels of MDA were significantly higher in group IR after ischemia reperfusion, but the activity of SOD and the concentrations of plasma insulin were remarkably lower （P〈0.05）. The hyperglycemic clamp test showed that the insulin secretion level, the glucose metabolism rate and the insulin sensitivity index in group IR were obviously lower than those in group C. Compared with those in group IR, the levels of insulin secretion, the GMR and the ISI were higher in group G. Conclusion Glutathione pretreatment has a protection against the disturbance of glucose metabolism during the hepatic ischemia/reperfusion injury （HIRI）. The mechanism of protection may be associated with its scavenging of oxygen free radical and inhibition of lipid peroxidation.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2011年第4期398-400,共3页
Journal of Clinical Anesthesiology
