摘要
目的：探讨视黄醇类化合物促胸腺细胞分化的分子机制。方法：选择幼年小鼠胸腺细胞为研究对象，采用免疫组化染色、Westernblot、流式细胞分析等实验方法，观察全反式视黄酸（ATRA）或视黄醇（Retinol）灌胃给药后对胸腺细胞分化成熟的影响，同时检测周期调控蛋白cyclinD1及相应基因表达变化情况。结果：ATRA和Retinol均可抑制胸腺细胞增殖活性，使胸腺组织中成熟功能T细胞比例增大；胸腺细胞cyclinD1、CDK4和RB基因表达呈时间剂量依赖性下调，而P16、P21蛋白表达上调，且ATRA作用优于Retinol。结论：RA可能经PRb、P16等多因素反馈调节cyclinD1和CDK4表达，抑或直接同其发生作用，进而影响胸腺细胞增殖分化的变化。
Objective: To explore the molecular mechanism of the role of cyclin D1 in the differentiationof thymocytes induced by all-trans retinoic acid and retinol. Methods: The expression of cyclin D1, CDK4,CKIs and PRb was determined with immunohistochemistry orimmunoblotting and the subgroups of murinethymocytes were analyzed with FCM 1 to 4 weeks after the young mice were treated with ATRA or retinol.Results: With the increase of ATRA content in vivo, functional T lymphocytes became a greater portion ofthymocytes. Both ATRA and retinol decreased the expression of cyclin D1, CDK4 and pRb but increased theexpression of P16 and P21. Conclusion: ATRA and retinol can stimu1ate the maturation of functional T lymphocytes in murine thymus. The changes in expression of cyclin D1, CDK4 and related genes might be themolecular mechanism of RA-induced differentiation of thymocytes.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第7期517-521,共5页
Journal of Third Military Medical University
