摘要
采用琼脂糖脂蛋白电泳分离脂蛋白结合甘油三酯（Ｔｇ）酶试剂呈色，扫描出各种脂蛋白区带中Ｔｇ所占比例，同血清总Ｔｇ浓度相乘计算各脂蛋白组份中Ｔｇ的含量。结果示本法与参考法比较相关系数：ＨＤＬ－Ｔｇ、ＶＬＤＬ－Ｔｇ、ＬＤＬ－Ｔｇ分别为０．８７３、０．９５５、０．９１２。方法的批内ＣＶ：ＨＤＬ－Ｔｇ、ＶＬＤＬ－Ｔｇ、ＬＤＬ－Ｔｇ分别为８．０％～１０．２％、３．８％～６．５％、４．３％～７．０％；批间ＣＶ分别为９．５％～１６．２％、６．８％～８．５％、４．８％～９．７％。平均回收率：ＨＤＬ－Ｔｇ、ＶＬＤＬ－Ｔｇ和ＬＤＬ－Ｔｇ分别为：８８．８％、９３．４％和９１．７％。冠心病组同相应的对照组比较ＬＤＬ－Ｔｇ（０．５０±０．３２ｖｓ０．２８±０．０９ｍｍｏｌ／Ｌ）及ＬＤＬ－Ｔｇ／ＬＤＬ－Ｃｈ比值（０．２１１±０．３１６ｖｓ０．０９５±０．０３３）均显著升高。该法简便易行，是精密准确定量各脂蛋白中Ｔｇ的理想方法。ＬＤＬ－Ｔｇ及ＬＤＬ－Ｔｇ／ＬＤＬ－Ｃｈ比值为冠心病有价值的诊断指标。
A simple method was developed for measurement of triglyceride(Tg)in lipoproteins. Lipoproteins separated by agarose gel electrophoresis.The Tg of bands was stained by specific enzymatic method(GPOTrinder)after electrophoresis.The relative amounts of Tg in lipoproteins were determined by densitometric scanning at 520 nm. Absolute triglyceride concentrations of the respective lipoprotein fractions were calculated from total Tg. Comparison of the electrophoresis method with a combined ultracentrifugation/precipitation method in 24 serum resulted in following correlation coefficients:HDLTg:=0873;VLDLTg:=0955;LDLTG:=0912The intraassay and interassay coefficients of variation were:8010238654370%;9516268854897%The average recovery was 888%934%917% respectively.This method is easy to perform,and is a precise and accurate technique for the determination of lipoprotein Tg. The results of 116 control subjects and 60 coronary heart disease(CHD)patients were shown:Total TGLDLTGLDLTG/LDLCH were valuable characler
出处
《临床检验杂志》
CAS
CSCD
北大核心
1999年第4期203-205,共3页
Chinese Journal of Clinical Laboratory Science
基金
江苏省应用基础研究课题
关键词
脂蛋白电泳
冠心病
甘油三酯
酶显色法
lipoprotein electrophoresis
triglyceride
coronary heart diseaseistics for CHD.
