摘要
目的：为探讨冲击波对豚鼠耳蜗毛细胞心钠素免疫反应的影响及与听阈阈移的相关性，对冲击波的致聋机理进行探讨。方法：对冲击波暴露后不同时间组采用免疫细胞化学技术（ＡＢＣ）结合图像分析和听性脑干反应测试技术进行观测。结果：冲击波暴露后２４ｈ内，内毛细胞（ＩＨＣ）中ＡＮＰ－ＩＲ阳性产物的光密度值较对照组明显增高（Ｐ＜０．０５），且与听阈阈移有明显的正相关性（ｒ＝０．９１８５，Ｐ＜０．０５）；而外毛细胞（ＯＨＣ）中心钠素免疫反应阳性产物变化不明显。结论：ＩＨＣ和ＯＨＣ中ＡＮＰ－ＩＲ的变化可能是冲击波对其损伤机制的不同表现。
Objective: To investigate the effect of shock wave on atrial natriuretic peptides immunoreactivity (ANPIR) in cochlear hair cells and its correlation with auditory threshold shift; to further study the mechanism of blast trauma causing deafness. Methods: After shock wave, different groups of guinea pigs were studied by ABC immunohistochemistry, image analysis techniques and auditory brainstem response. Results: The ANPIR optic density was higher than that of control in inner cells. The change of ANPIR optic density was directly correlated with ABR threshold shift (r=0.9185,P<0.05). There was no change of ABR and ANPIR optic density in outer hair cells. Conclusion: The change of ANPIR in inner cells may be a pathological manifestation of shock wave.
出处
《中国耳鼻咽喉颅底外科杂志》
CAS
1999年第2期69-71,共3页
Chinese Journal of Otorhinolaryngology-skull Base Surgery
