摘要
目的 从肺泡表面活性物质的变化探讨新生儿猝死综合征的病因。方法 用薄层色谱法对 10例新生儿猝死综合征和 10例对照新生儿 (死于非呼吸系疾病 )的全肺表面活性物质作定性和定量检测。结果 全肺表面活性物质定性分析得到 11个组分 :溶血磷脂酰胆碱、神经鞘磷脂、磷脂酰胆碱、磷脂酰丝氨酸、磷脂酰肌醇、磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、磷脂酸、胆固醇和中性脂类。定量分析显示 :猝死组全肺表面活性物质含量为 ( 8.9± 1.0 ) mg/g肺湿重 ,显著少于对照组的( 12 .6± 1.4 ) mg/g肺湿重 ( P<0 .0 1)。猝死组的磷脂酰胆碱 ( 4 9.4± 2 .9) %和磷脂酰甘油 ( 2 .6±0 .7) %比例下降 ,而对照组分别为 ( 61.5± 3 .0 ) %和 ( 4 .3± 1.5 ) % ( P<0 .0 1 )。结论 猝死新生儿生前在肺泡表面活性物质代谢方面存在着严重缺陷 :合成数量的减少和合成品种的比例失调。这种缺陷是新生儿猝死综合征的一个重要病因。
Objective To find pathogenies of sudden infant death syndrom (SIDS) from changes of pulmonary surfactant Methods By means of thin layer chromatography technique,surfactant in whole lung specimens of 10 infants with SIDS and 10 control infants without SIDS,dying from nonrespiratory disease,were examined qualitatively and quantitatively Results Eleven components in pulmonary surfactant of the whole lung specimens were examined qualitatively,including lysophosphatidylcholine,sphingomyelin,phosphatidylcholine,phosphati dylser in,phosphatidylinosito l,phosphatidylcholine,phosphatidylglycerol,diphosphatidylglycerol,phosphatidic acide,cholestrol and neutral lipids Quantitative examination showed that the amount of surfactant of whole lung specimens in the sudden death group (8 9±1 0mg/g wet lung weight) was significantly less than in the control group (1 26±1 4mg/g wet lung weight) (P<0 01) Qualitative variance showed that the percentages of phosphatidylcholin (49 4%±2 9%) and phosphatidylglycerol (2 6%±0 7%)decreased markedly in sudden death group compared with in control group(61 5%±3 0% and 4 3%±1 5%)(P<0 01) Conclusion Before death there is serious defect on metabolism of pulmonary surfactant in sudden death infant,with the amount decreasing,and the ratio of its components being disturbed,which is one of the important pathogenies of SIDS
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
1999年第7期423-425,共3页
Chinese Journal of Anesthesiology
关键词
猝死
肺泡表面活性剂
新生儿
Sudden infant death Pulmonary surfactants
