摘要
目的检测结肠癌组织中Twist蛋白的表达情况,探讨Twist蛋白表达与结肠癌发生、发展的关系。方法采用链霉亲和素-生物素-过氧化物酶复合物(SABC)免疫组织化学法检测70例结肠癌及切端结肠组织Twist蛋白表达水平,结合临床病理资料,进行统计学分析。结果结肠癌组织中Twist蛋白表达阳性率(72.9%)显著高于切端结肠组织(12.9%),差异有统计学意义(P<0.01)。不同TNM分期的结肠癌组织中Twist蛋白表达水平(Ⅰ期10.0%、Ⅱ期66.7%、Ⅲ期88.0%、Ⅳ期100.0%)比较,差异有统计学意义(P<0.01);其中Ⅲ、Ⅳ期结肠癌组织Twist蛋白表达水平显著高于Ⅰ期,Ⅳ期又显著高于Ⅱ期,差异均有统计学意义(P<0.008)。结论结肠癌组织中Twist蛋白表达升高,且会随着肿瘤的侵袭和转移而升高。提示Twist蛋白表达在结肠癌发生、侵袭等生物学过程中可能发挥着一定作用,在一定程度上可作为检测恶性肿瘤进展的生物学指标之一。
Objective To explore the relationship of Twist protein expression to the development of colon carcinoma.Methods Streptavidin biotin complex（SABC） immunohistochemistry was performed to detect the Twist protein expression in 70 cases of colon carcinoma tissues and cutting edge tissues.Results The Twist protein expression was higher in colon carcinoma tissues than in cutting edge（72.9% vs 12.9%,P0.01）.There was significant difference in Twist protein expression between different TNM stages of colon carcinoma tissues（stage Ⅰ 10.0%,stage Ⅱ 66.7%,stage Ⅲ 88.0%,stage Ⅳ 100.0%,P0.01）;and the level of Twist protein expression was significantly higher in stages Ⅲ and Ⅳ than in stages Ⅰand Ⅱ（P0.008）.Conclusion Twist protein expression in colon carcinoma tissues increases with the invasion and metastasis,suggesting that Twist protein expression may play a certain role in such biological processes as development and invasion of colon carcinoma and can be used as one of biological indicators detecting progress of malignant tumors.
出处
《中国全科医学》
CAS
CSCD
北大核心
2011年第12期1311-1314,共4页
Chinese General Practice
