摘要
目的探讨雷帕霉素对膀胱癌T24细胞增殖和侵袭能力的影响。方法将T24细胞贴壁培养于10%胎牛血清、100U/mL青霉素、100μg/mL链霉素的RPMI1640培养液中,37℃、5%CO2饱和湿度培养箱中培养。取对数生长期细胞,随机分为处理组(加入浓度为10～1000nmol/L的雷帕霉素)和对照组(不加药物),每天更换RPMI1640培养液。采用MTT法检测各组细胞生长抑制率,transwell小室法检测细胞体外侵袭能力的变化。结果雷帕霉素对T24细胞有增殖抑制作用,呈剂量依赖关系。transwell法检测结果显示,雷帕霉素处理后的T24細胞体外侵袭能力明显下降,与雷帕霉素呈剂量依赖性。结论雷帕霉素具有抑制膀胱癌T24细胞增殖和侵袭能力的作用。
Objective To investigate the effects of rapamycin on proliferation and invasion of human bladder cancer cell T24.Methods T24 cells were maintained in RPMI-1640 containing 10% fetal bovine serum,penicillin（100 U/mL）and streptomycin（100 μg/mL）.Cells were cultured at 37 ℃ in a 5% CO2 incubator.T24 cells in logarithmic growth phase were selected and randomly divided into experimental group[treated with various concentrations of rapamycin solution（10 to 1 000 nmol/L）]and control group（treated with RPMI 1640）.Growth inhibition rates of the tumor cells were measured by MTT colorimetry.The changes of invasiveness of cells were measured by transwell chamber assay.Results Rapamycin inhibited proliferation of T24 tumor cells in a concentration-dependent manner and inhibited invasiveness of T24 cells in transwell chamber assay.Conclusion Rapamycin inhibited growth and invasiveness of bladder carcinoma cells.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2011年第4期301-303,共3页
Journal of China Medical University
基金
辽宁省科技计划项目(2009225008-14)
