摘要
目的:探讨紫草素对血管平滑肌细胞(VSMC)化疗性损伤产生预防性保护及治疗作用的机制。方法:培养兔血管平滑肌细胞,以硫酸长春新碱建立化疗性损伤模型,采用噻唑兰(MTT)法测定细胞活性,并测定细胞乳酸脱氢酶(LDH)和细胞内游离Ca2+浓度,使用流式细胞术比较各组间细胞周期的改变。结果:紫草素用于预防或治疗时,实验组的细胞活性、LDH的水平及细胞内游离Ca2+浓度与模型组比较差异均有极显著性(P<0.01),与空白组相比接近,差异无显著性(P>0.05);紫草素可消除化疗药物对细胞有丝分裂阻滞作用,使G2期细胞比例恢复正常,单药时可提高正常细胞S期比例。结论:紫草素对VSMC化疗损伤具有预防性保护作用和损伤后的修复作用。
OBJECTVE To explore the mechanism of protect effect shikonin on vascular smooth muscle cells damaged by chemotherapy. METHODS Chemotherapy injury model was made of rabbit vascular smooth muscle cells with the vincristine sulfate, the changes of cytoactive by shikonin was measured by methyl thiszolyl tetrazolium（MTT）, and the level of lactate dehydrogenase（LDH） and free Ca2＋ in VSMC were determined, and the changes of VSMC cell cycle between all groups was compared by the flow cytometry（FCM）. RESULTS There was significant difference in cytoactivty and LDH level and free Ca2＋ between experimental group and model group（P〈0. 01 or P〈0. 05） ,and the data of experimental group got close to the control group, there was no signficant difference; Shikonin could abscise the blocking on the mitosis of chemotherapeutics, put right the ratio of G2 phase,and the ratio of S phase when shikonin used alone. CONCLUSION Shikonin had obviously prophylactic protection on demaged and regeneration on injury of VSMC coused by chemotherapy.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2011年第8期640-643,共4页
Chinese Journal of Hospital Pharmacy
基金
天津市卫生局资金课题(编号:2007052)
关键词
紫草素
血管平滑肌细胞
化疗性损伤
硫酸长春新碱
细胞周期
shikonin
vascular smooth muscle cells
chemotherapeutic damage
vincristine sulfate
cell cycle
