摘要
目的 研究不同肠系膜上动脉注射液对缺血小肠功能改变的影响。方法 制成兔小肠缺血再灌注模型。分别观察缺血即刻、完全阻断６０ ｍ ｉｎ 和再灌注３０ ｍ ｉｎ 时小肠细胞膜Ｎａ＋ －Ｋ＋ ＡＴＰ酶、Ｃａ２＋ －ＡＴＰ酶活性和小肠线粒体游离Ｃａ２＋ 浓度。结果 ①完全阻断６０ ｍ ｉｎ 时，异搏定组Ｎａ＋ －Ｋ＋ ＡＴＰ酶活性（１２．４４５±３．２１９） μＭＰｉ／（ｍ ｇ·ｈ）、Ｃａ２＋ －ＡＴＰ酶活性（１０．９７３±３．０３３） μＭＰｉ／（ｍ ｇ·ｈ）和Ｃａ２＋ 浓度（２．９７６±０．４１０） ｎｍ ｏｌ／ｍ ｇ，能量合剂组Ｃａ２＋ －ＡＴＰ酶活性（１０．３７６±２．８４４） μＭＰｉ／（ｍ ｇ·ｈ），优于对照组（Ｐ＜ ０．０１）；②再灌注３０ ｍ ｉｎ 时异搏定组和能量合剂组两酶活性［分别为（１０．７７５±３．２２７）、（８．４４４±２．１２８） μＭＰｉ／（ｍ ｇ·ｈ）和（１０．１１８±３．４２５）、（７．６３４±１．４０４） μＭＰｉ／（ｍ ｇ·ｈ）］和Ｃａ２＋ 浓度［分别为（２．４０１±０．３２３）ｎｍ ｏｌ／ｍ ｇ， （３．８４７±０．６１０）ｎｍ ｏｌ／ｍ ｇ］均高于对照组（Ｐ＜ ０．０１）。结论 异搏定和能量合剂可以显著改善缺血小肠的?
Objective\ The purpose of this study is to probe the changes of the functions of the ischemic small bowel with different perfusion solutions via supper mesenteric artery (SMA). Methods\ Saline, verapamil in saline, and energy compound solution were injected respectively via SMA in rabbits before the artery was tied to produce ischemia/reperfusion (I/R) injury. The activities of Na\++ K\++ ATPase and Ca\+\{2+\} ATPase as wellas mitochondria free calcium concentrations in mucosa cells were monitored in the different groups. Results\ The activities of Na\++ K\++ ATPase, Ca\+\{2+\} ATPase in plasma membrance declined in normal saline perfusion group after ischemia (\%P\%<0.01); Compared to the control group, verapamil and energy compounds solutions significantly improved the two ATPase activities (\%P\%<0.01); The free calcium concentration in mitochondria increased in control group but declined in verapamil group after ischemia (\%P\%<0.01). Conclusion\ The verapamil and energy compounds solutions show the protective effects on the functions of ischemic small intestine.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
1999年第6期469-471,共3页
Journal of Nanjing Medical University(Natural Sciences)
关键词
肠系膜上动脉
小肠缺血
再灌注
异搏定
ATP酶
supper mesenteric atery
ischemia/reperfusion
verapamil
ATPase
Ca\+\{2+\}
rabbits
