摘要
目的 研究ｃｙｃｌｉｎ Ｄ１／ｐ１６－ｐＲＢ路径在脑胶质瘤中的异常情况，并对其意义进行分析。方法 用免疫组化法对２３例脑胶质瘤（包括１４ 例星形细胞瘤和９例胶质母细胞瘤）及６ 例正常脑组织中３ 种因子的表达进行检测，并分析了实验结果与肿瘤分型的关系以及各个因子在此路径中的作用。结果 ２３ 例肿瘤中有１７ 例ｃｙｃｌｉｎ Ｄ１ 过度表达（７３．９％ ），１３ 例ｐ１６ 和８ 例ｐＲＢ缺失（５６．５％ 和３４．８％ ），３ 种因子的异常均与肿瘤分型有关（Ｐ＜ ０．０１）。ｃｙｃｌｉｎ Ｄ１／ｐ１６－ｐＲＢ路径作为整体在肿瘤组织中的异常率为８２．６％ （１９／２３），与正常脑组织（１／６， １６．７％ ）相比有显著性差异（Ｐ＝ ０．００６）；另外星形细胞瘤（１０／１４， ７１．４％ ）和胶质母细胞瘤（９／９， １００％ ）之间并无统计学差别（Ｐ＝ ０．１２４），说明此路径异常与肿瘤发生有关而与分型无关。３种因子的异常两两之间无显著性相关（Ｐ＞ ０．０５）。结论 ｃｙｃｌｉｎ Ｄ１／ｐ１６－ｐＲＢ路径与脑胶质瘤发生关系密切，可以作为探讨肿瘤病因、诊断和治疗的重要靶位。
Objective\ Cyclin D1/p16 pRB pathway was known as an important cell cycle regulator at the G1→S checkpoint. This experiment will study the alterations of this pathway in gliomas and its effects. Methods\ The expressions of cyclin D1, p16 and pRB were screened in 23 gliomas (including 14 astrocytomas and 9 glioblastomas) and 6 normal brain tissues by immunohistochemical method. We analyzed the relationship between the pathway abnormalities and tumor types. The association of the three factors was also studied. Results\ In 23 gliomas, 17 has cyclin D1 overexpression (73.9%), 13 had p16 and 8 had pRB deletion (56.5% and 34.8% each); Abnormalities of these proteins were closely related to tumor types ( P <0.01). The alteration of cyclin D1/p16 pRB pathway as a unit was seen in 82.6%(19/23) of the cases and had marked difference with normal brain tissues (1/6, 16.7%)( P =0.006); However there was no statistical significance between astrocytomas (10/14, 71.4%) and glioblastomas (9/9, 100%) indicating that the pathway abnormalities was only associated with tumorigenesis but not with tumor types. We also found no differences in cyclin D1, p16, or pRB abnormal expression ( P >0.05). Conclusion\ Cylcin D1/p16 pRB pathway is closely related with tumorigenesis and malignant transformation. It is probably an important target in studying the etiology, diagnosis and gene therapy of tumors.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
1999年第6期463-466,共4页
Journal of Nanjing Medical University(Natural Sciences)
