摘要
目的:研究褪黑素对小鼠2-细胞期胚胎发育的影响。方法:在培养液中添加不同浓度褪黑素,在不同的作用时间点,观察并记录小鼠胚胎囊胚率、孵出率,研究其变化情况。结果:不同浓度褪黑素对小鼠2-细胞期胚胎存在双向作用,在10-13-10-5M之间促进细胞囊胚形成和孵出,在10-9M时促进效应达到最高,而在10-3M时则呈现出一定的毒性作用。结论:褪黑素对小鼠胚胎发育影响与褪黑素浓度密切相关。
Objective：To investigate the effects of melatonin on the development of mice 2-cell embryos.Methods：The effects of various concentrations of melatonin（10-13-10-3M）on the development of mouse embryos were detected.The blastocyst rate and hatching rate of mouse embryos were recorded after treated by different concentrations of melatonin.Results：Melatonin could improve the devel-opment of mice 2-cell embryo in a bi-phasic manner.The rates of blastocyst formation and hatched blastocysts increased by melatonin at 10-13-10-5M,and the maximum effects arrived at 10-9M.However,the toxic effects appeared at 10-3M.Conclusions：Melatonin affect the development of mice embryos depending the concentrations of melatonin.
出处
《现代生物医学进展》
CAS
2011年第6期1072-1074,共3页
Progress in Modern Biomedicine
