摘要
目的:建立疟疾的实时荧光PCR检测方法并用于疟疾的快速检测。方法:设计了疟原虫通用型实时荧光PCR检测的引物和探针,建立了疟疾的实时荧光PCR检测方法。对40份疟疾镜检阳性样本和20份镜检阴性样本进行实时荧光PCR检测,并和巢式PCR方法检测结果进行了比较。结果:疟疾实时荧光PCR检测速度快,20分钟即可完成。40份镜检阳性样本的荧光PCR检测均为阳性,2份样本的巢式PCR结果为阴性;20份镜检阴性样本中有3份样本为实时荧光PCR阳性,其余17份样本为实时荧光PCR阴性,与疟疾巢式PCR检测结果相同。结论:该实时荧光PCR方法检测速度快,特异性强,准确性高,阳性率高,不易漏检,适用于疟疾的快速检验,这对防止该病在国境口岸的传入提供了技术依据。
Objective：To establish real-time fluorescent PCR detection method for rapid detection of malaria.Methods：Universal primers and probes were designed.Forty positive samples and twenty negative samples tested by microscopic examination were detected Plasmodium by real-time fluorescent PCR.Their results were compared with that of nested PCR.Results：Real-time fluorescence PCR detection of malaria was fast and the reaction time only took 20 minutes.All of forty microscopy-positive samples were positive for fluorescent PCR detection,and two samples were negative by nested PCR.Three of twenty microscopy-negative samples were positive for real-time fluorescence PCR and the remaining seventeen samples were negative,which was the same as results of nested PCR detection.Conclusion：The real-time fluorescent PCR is suitable for rapid detection of malaria because of its fast speed,strong specificity,high accuracy and low missing rate.It provides a technical basis to prevent disease from spreading into our country in the border.
出处
《中国卫生检验杂志》
CAS
2011年第3期625-627,共3页
Chinese Journal of Health Laboratory Technology
基金
广东检验检疫局科技计划项目(2007GDK32)
