摘要
目的通过RT-PCR方法对2009年宁波市狂犬病病毒分离株(NB0901)的N基因扩增并测序,了解宁波地区狂犬病病毒的流行特点。方法运用RT-PCR方法和序列测定方法获得NB0901株的N基因序列,并在核苷酸和氨基酸水平与疫苗株(3aG和CNT-1)进行同源性比较,同时进行遗传进化分析。结果狂犬病病毒NB0901株与疫苗株的核苷酸和氨基酸序列同源性分别在86.6%～90.1%和95.5%～98.8%之间。NB0901株N蛋白的抗原表位与疫苗株相比后发现在NⅠ和NⅡ抗原表位存在突变。而在Th细胞表位上,NB0901株与CNT-1株完全一致,与3aG株相比变异较大。进化结果表明NB0901株属于狂犬病病毒Ⅰ群中的A亚型。结论狂犬病病毒NB0901株和当前疫苗株虽同属于Ⅰ群,但是与疫苗株相比存在差异,从氨基酸序列水平上分析,CNT-1株较3aG株更能有效保护流行毒株感染。
Objective The nucleoprotein structural gene of the Rabies virus NB0901 strain isolated in Ningbo city in 2009 was amplified and sequenced by RT-PCR to get an insight into the epidemiological characteristics of rabies in this area.Methods The N gene sequence of the NB0901 strain was obtained using RT-PCR and sequence analysis.The homology of the gene with vaccine strains,3aG and CNT-1,was analyzed at the nucleotide and amino acid level,respectively,followed by phylogenetic analysis.Results The homology between the NB0901 strain and the vaccine strains ranged from 86.6%-90.1% to 95.5%-98.8%,respectively,at the level of nucleotide and amino acids.By comparison with the vaccine strains,mutations were found at NⅠ and NⅡ antigen epitopes of the NB0901 strain.Epitopes of the T helper cells were identical between the NB0901 strains and CNT-1 strains,whereas those in 3aG had remarkably mutated,suggesting that the NB0901 strain belonged to subtype A of groupⅠof Rabies virus.Conclusion Though NB0901 and the current vaccine strains belonged to the same group,this strain was partly different from the latter.Based on the analysis at the amino acid level,CNT-1 strains would be more effective in protecting infection of this strain than 3aG strains.
出处
《中国媒介生物学及控制杂志》
CAS
CSCD
2011年第2期148-150,共3页
Chinese Journal of Vector Biology and Control
关键词
狂犬病病毒
核蛋白
序列分析
Rabies virus
Nucleoprotein
Sequence analysis
