摘要
[目的]构建黄鳝抗菌肽hepcidin基因大肠杆菌表达载体。[方法]根据Genbank中黄鳝hepcidin基因序列设计引物进行PCR扩增,将测序正确的基因片段连接入载体pET-28a中。[结果]以黄鳝cDNA为模板,扩增出hepcidin基因片段,长度为291 bp,连接到pET-28a上。经PCR扩增、双酶切验证,证实成功构建原核表达载体。[结论]成功构建了黄鳝hepcidin基因大肠杆菌表达载体。
[Objective]To construct a E.coli expression vector of hepcidin gene from rice field eel.[Method]According to hepcidin gene sequence of rice field eel reported by genbank,primers were designed,and PCR was performed to amplify the hepcidin gene.Then the purified fragment was cloned into pET-28a vector.[Result] Hepcidin gene was amplified from the cDNA of rice field eel.The gene fragment was 291 bp,and was cloned into pET-28a vector.After analysis of restriction endonuclease digestion and PCR,the constructed expression vector was proved to be correct.[Conclusion]The expression vector of hepcidin gene of rice field eel was constructed successfully.
出处
《安徽农业科学》
CAS
北大核心
2011年第9期5245-5246,共2页
Journal of Anhui Agricultural Sciences
基金
长江大学2009年大学生创新性实验计划项目(0901)
关键词
黄鳝
抗菌肽
基因
原核表达载体
Rice field eel
Anti-bacterial peptides
Hepcidin Gene
E.coli expression vector
