摘要
[目的]优化农杆菌介导番茄"美粉1号"遗传转化体系。[方法]以番茄美粉1号无菌苗的子叶为外植体,通过农杆菌介导法对其遗传转化效率进行了优化,建立高效番茄子叶农杆菌介导的遗传转化体系。[结果]外植体在MS+2.0 mg/L6-BA+0.5 mg/L IAA进行2 d的预培养后,用农杆菌EHA105(浸染浓度为OD=0.4)浸染5 min,转化效率最高;经过PCR检测初步证明nptⅡ基因已整合到番茄再生植株中。[结论]该研究结果为优良基因导入番茄品种美粉1号奠定了基础。
[Objective] The aim of this study was to carry out study on the optimization of Agrobacterium mediated genetic transformation system of tomato Meifen No.1.[Method] The cotyledon of tomato cultivar Meifen No.1 was used as the explant,and the Agrobacterium mediation method was used to optimize its genetic transformation efficiency so as to establish the efficient Agrobacterium mediated genetic transformation system of tomato cotyledon.[Result] The highest transformation efficiency was obtained when the explants were cultivated for 2 d on MS ＋ 2.0 mg/L 6-BA＋ 0.5 mg/L IAA medium and then infected with Agrobacterium EHA105（OD = 0.4） for 5 min;it was proved by PCR analysis that the target nptII gene had been integrated into the genome of regenerated plants.[Conclusion] The result in this study had provided basis for the transfer of valuable genes into tomato Meifen No.1.
出处
《安徽农业科学》
CAS
北大核心
2011年第9期5107-5108,5117,共3页
Journal of Anhui Agricultural Sciences
基金
中国农业大学植物生理学与生物化学国家重点实验室开放课题(SKLPPBKF09011)
关键词
根癌农杆菌
番茄
遗传转化
Agrobacterium tumefaciens
Tomato
Transgenic transformation
