摘要
目的探讨姜黄素联合美沙拉嗪治疗溃疡性结肠炎小鼠的疗效及作用机制。方法 50只小鼠分成5组:正常组、模型组、姜黄素联合美沙拉嗪治疗组、姜黄素治疗组和美沙拉嗪治疗组,采用传统的葡聚糖酸钠法制备小鼠溃疡性结肠炎模型。自造模成功次日,姜黄素联合美沙拉嗪治疗组予姜黄素灌肠及美沙拉嗪灌胃治疗;姜黄素治疗组仅予姜黄素灌肠治疗;美沙拉嗪治疗组予美沙拉嗪灌胃治疗;正常组及模型组均予9 g.L-1盐水灌胃。以上均为每日1次,共10 d。给药10 d后,观察其结肠病理组织学变化,并采用流式细胞术检测其结肠上皮细胞凋亡率,采用ELISA法测定小鼠血清IL-2、IL-10水平。结果姜黄素联合美沙拉嗪治疗组结肠指数、结肠损伤评分、病理组织学评分及细胞凋亡率较模型组均明显下降(Pa<0.01)。与姜黄素治疗组和美沙拉嗪治疗组比较,姜黄素联合美沙拉嗪治疗组结肠损伤评分、病理组织学评分及细胞凋亡率有所下降,且差异均有统计学意义(Pa<0.05);与模型组比较,姜黄素联合美沙拉嗪治疗组、姜黄素治疗组和美沙拉嗪治疗组血清IL-10水平均显著下降,血清IL-2水平显著升高(Pa<0.01)。与姜黄素治疗组和美沙拉嗪治疗组比较,姜黄素联合美沙拉嗪治疗组血清IL-10水平显著下降,血清IL-2水平显著升高(Pa<0.05)。结论姜黄素联合美沙拉嗪治疗小鼠溃疡性结肠炎,效果较单用美沙拉嗪好,姜黄素联合美沙拉嗪治疗小鼠溃疡性结肠炎在降低IL-10、升高IL-2水平方面较单用美沙拉嗪显著。
Objective To investigate the effect of curcumin combined with mesalazine on the treatment of ulcerative colitis in mice and its mechanism.Methods Fifty mice were divided into 5 groups：normal group,model group,curcumin combined with mesalazine treatment group,curcumin treatment group and mesalazine treatment group.The mouse models with ulcerative colitis were copied by dextran sulfate so-dium traditional method.Since the model was successfully constructed,the next day,curcumin combined received mesalazine treatment group received curcumin enema treatment and mesalazine orally fed of each;curcumin treatment group received curcumin enema treatment;mesalazine treatment group received mesalazine orally fed;normal group and model group were treated with correspondingly 9 g·L-1 saline.All once every day,a total for 10 d.Ten days after administration,the pathological changes were observed,and flow cytometry was used to detect the apo-ptosis of colonic epithelial cells,ELISA was used to detect the serum IL-2 and IL-10 levels.Results The colonic damage scores,histolo-gical scores and the apoptosis rate in curcumin combined with mesalazine treatment group were significantly lower than those in model group（Pa0.01）.Compared with the curcumin treatment group and mesalazine treatment group,the injury score,histological score and the apoptosis rate in curcumin combined with mesalazin treatment group decreased,and the differences were statistically significant（Pa0.05）.Compared with model group,IL-10 levels decreased and IL-2 levels increased significantly in curcumin combined with mesalazine treatment group and the curcumin treatment group and mesalazine treatment group（Pa0.01）.Compared with the curcurnin treatment group and mesalazine treatment group,IL-10 levels decreased and IL-2 levels increased significantly in curcumin combined with mesalazine treatment group（Pa0.05）.Conclusion Curcumin combined with mesalazine therapy is better than using mesalazine alone on mice with ulcerative colitis,and also effective in decreasing IL-10 level and increasing IL-2 level compared with using mesalazine alone.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2011年第7期532-534,共3页
Journal of Applied Clinical Pediatrics
