摘要
目的:建立磺丁基-β-环糊精中有关物质β-环糊精检测及含量测定的高效液相色谱分析。方法:采用Waters XTerraMs-C18(4.6 mm×250 mm,5μm)柱;流动相:甲醇-水(8∶92);检测器:示差折光率检测器;流速:0.8 mL·min-1;柱温:25℃。结果:在所选定的色谱条件下,磺丁基-β-环糊精与β-环糊精能分离完全;β-环糊精在0.1～1.6 mg·mL-1内,峰面积与浓度线性关系良好,r=0.9999;最低检测限为2μg。结论:方法简便,准确,专属性强,可用于磺丁基-β-环糊精中的β-环糊精检测及含量测定质量控制。
Objective：To develop a HPLC method for the determination of sufobutylether-β-cyclodenxtin and β-cyclodenxtin for quality control.Methods：The separation was carried out on a Waters XTerra Ms-C18 column（4.6 mm×250 mm,5 μm）,using a mixture of methanol-water（8∶92）as the mobile phase at a flow rate of 0.8 mL·min-1.The Refractive Index Detection,the column temperature was 25 ℃.Results：Under the established chromatographic conditions,sufobutylether-β-cyclodenxtin and β-cyclodenxtin were separated completely.The standard curve was linear within the range of 0.0625-1.5 mg·L-1 with the correlation coefficient of 0.9999,the limit of detection was 0.1 mg.Conclusion：The established method is simple,accurate,specific and can be used for quality control of sufobutylether-β-cyclodenxtin.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2011年第4期749-751,共3页
Chinese Journal of Pharmaceutical Analysis
