摘要
目的 通过静脉注入抗ＣＤ４ 、ＣＤ８ 免疫毒素及供体可溶性抗原诱导胰岛移植物免疫耐受。方法 供、受体分别为Ｗｉｓｔａｒ 大鼠和ＳＤ大鼠， 移植前１４ 天、７ 天分别将免疫毒素各２００ μｇ， 供体可溶性抗原５００ μｇ 经静脉注入受体， 然后将供体５００ 个胰岛移植于受体（ 糖尿病大鼠）左侧肾包膜下。结果 用免疫毒素及供体可溶性抗原联合处理组胰岛移植物存活时间显著延长（ Ｐ＜ ０ ．０１） ， 而单独应用抗ＣＤ４ 、ＣＤ８ 免疫毒素或供体可溶性抗原组仅能获得胰岛移植物存活时间轻度延长。结论 抗ＣＤ４、ＣＤ８
Objective To induce islet grafting tolerance by intravenous injection of anti CD4, anti CD8 immunotoxins and donor soluble antigen.Methods 14 days or 7 days prior to transplantation, the immunotoxon 200?μg respectively, and donor soluble antigen 500?μg were injected intravenously into the recipients, then 500 donor islets were translanted under the left renal subcapsular space of diabetes recipients (SD rats). Results The islet grafting survival time that pretreated with immunotoxon and donor soluble antigen was over 60 days ( P < 0.01 ). The immunotoxins or donor soluble antigen treatment alone only slightly prolonged the graft survival.Conclusion The anti CD4, anti CD8 immunotoxins combined with donor soluble antigen can induce donor specific immune tolerance.
出处
《中华器官移植杂志》
CAS
CSCD
1999年第4期218-220,共3页
Chinese Journal of Organ Transplantation
基金
国家自然科学基金
博士后基金
关键词
免疫毒素
供体
可溶性抗原
胰岛移植
免疫耐受
Immunotoxin Donor soluble antigen Islet/transplantation Immune tolerance
