摘要
目的观察ＨＰＶ１６，１８Ｅ６在人宫颈癌中的表达并说明Ｅ６单抗的应用价值。方法采用Ｓ－Ｐ免疫组化染色法，检测５０例宫颈鳞状细胞癌，３０例慢性宫颈炎及３０例正常宫颈组织中ＨＰＶ１６，１８ Ｅ６蛋白的表达。结果宫颈癌组中Ｅ６表达的阳性率为７０％（３５／５０），慢性宫颈炎组中Ｅ６阳性率为６．７％（２／３０），４例腹股沟淋巴结转移癌Ｅ６阳性率为７５％（３／４），正常宫颈组中Ｅ６表达均为阴性。Ｅ６的表达与肿瘤的分化程度无关（Ｐ＞０．０５），与肿瘤的良恶性有显著差异（ Ｐ＜ ０．０１）。结论 ＨＰＶ１６，１８感染与宫颈癌病因学密切相关，Ｅ６单抗可作为预测ＨＰＶ１６，１８感染及早期诊断宫颈癌的标记之一。
Objective To investigate the expression of human papillomavirus (HPV) 16, 18 & E6 of cervical sdquamous cell carcinoma and to assess the practicl value of HPV16,18 E6 antibody. Method Immunohistochemistry S-P method was used to examine HPV16,18 protein expressions in 50 cases of squamous cell careinoma of uterine cervix, 30 cases of chronic cervicitis and 30 cases of nounal cervical epidermal tissue. Result 35 cases of carcinoma and, 2 cases of chronic cervicitis and 3 cases of metastases carcinomas showed positive staining. None of the normal epithelium specimens gave positive results. The difference between malignant and benign groups was significant, but nodifference in the grades of differentiation in cervical squamous cell carcinoma. Conclusion A close relation between high risk HPV16,18 infection and cervical carcinogensis was found. HPV16,18 E6 antibody could be a good indicator for HPV16,18 infection and early diagnosis of cervical carcinoma.
出处
《河南肿瘤学杂志》
1999年第5期353-355,共3页
Henan Journal of Oncology
关键词
宫颈肿瘤
癌
免疫组织化学
表达
cervix neoplasms , carcinoma,Immunhistochemistry
