摘要
目的 探讨慢性阻塞性肺疾病（ Ｃ Ｏ Ｐ Ｄ） 和支气管哮喘患者气道分泌物中，感觉神经肽降钙素基因相关肽（ Ｃ Ｇ Ｒ Ｐ） 释放在气道慢性炎症性疾病发病中的作用。方法 选择稳定期 Ｃ Ｏ Ｐ Ｄ 患者１９ 例、支气管哮喘患者１４ 例、正常对照组１０ 名，通过面罩吸入超声雾化的４ ％ 高张盐水诱导痰液，将获得的诱导痰涂片进行瑞氏姬姆萨染色计数细胞成分的变化，用放射免疫方法测定诱导痰中 Ｃ Ｇ Ｒ Ｐ免疫反应性物质（ Ｃ Ｇ Ｒ Ｐ Ｌ Ｉ） 的含量改变，并进行 Ｃ Ｇ Ｒ Ｐ Ｌ Ｉ与一秒钟用力呼气容积（ Ｆ Ｅ Ｖ１） 的相关性分析。结果 诱导痰中 Ｃ Ｇ Ｒ Ｐ Ｌ Ｉ含量在 Ｃ Ｏ Ｐ Ｄ 组为（１５９７ ±２１５）ｎｇ／ Ｌ，支气管哮喘组为（１８７９ ±３９１）ｎｇ／ Ｌ，与正常对照组（２３６ ±０３５）ｎｇ／ Ｌ 比较，差异有显著性（ Ｐ＜ ００５） ，而且与 Ｆ Ｅ Ｖ１ 呈显著负相关（ ｒ＝ － ０５０ 和－ ０６１ ， Ｐ 均＜ ００５） 。 Ｃ Ｏ Ｐ Ｄ 患者诱导痰中中性粒细胞百分比为（６４９ ±２９） ％ ，与支气管哮喘患者（２６５ ±１５） ％ 和正常对照组（２５９ ±２６） ％ 比较，差异有显著性（ Ｐ＜ ００１） ；支气管哮喘?
Objective To explore the role of sensory neuropeptide calcitonin gene related peptide (CGRP) in the pathogenesis of chronic airway inflammatory diseases COPD and bronchial asthma Methods Patients with COPD ( n =19), bronchial asthma ( n =14), all were in stable stage and 10 normal volunteers were examined After hypertonic saline inhalation challenge in all subjects, CGRP LI concentration in the induced sputum was measured by radioimmunoassay Cellular content was assayed by microscopic analysis, the relation between CGRP LI level and FEV 1 value was calculated by liner regression Results The sputum CGRP concentrations in patients with COPD and patients with asthma were (15 97±2 15)ng/L, (18 79±3 91)ng/L, respectively, both were significantly higher than those in normal volunteers (2 36±0 35)ng/L Moreover, CGRP concentrations in induced sputum in each disease group were correlated with the degree of airflow obstruction, r = -0 50 and -0 61, respectively ( P <0 05) The percentage of neutrophil cell count (64 9±2 9)% was significantly higher in patients with COPD ( P <0 01), while the percentage of eosinophil cell count (5 8±0 5)% was increased in patients with asthma ( P <0 01) Conclusions The data suggested that CGRP release may participate in the chronic inflammation of patients with COPD and bronchial asthma
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1999年第9期558-561,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家杰出青年科学基金
国家教委博士点基金
关键词
阻塞性肺疾病
哮喘
CGRP
Calcitonin gene related peptide Pulmonary disease, obstructive Asthma
