摘要
目的：建立人端粒酶催化亚基蛋白质 Ｒ Ｎ Ａ（ｈ Ｔ Ｒ Ｔ Ｒ Ｎ Ａ）逆转录聚合酶链反应（ Ｒ Ｔ Ｐ Ｃ Ｒ），进行恶性肿瘤细胞和胃癌组织检测，探讨其在癌变中的作用，提供一种癌症检测的新方法。方法：制备癌细胞株和胃癌组织端粒酶提取液和总 Ｒ Ｎ Ａ，运用端粒重复扩增法（ Ｔ Ｒ Ａ Ｐ）和 ｈ Ｔ Ｒ Ｔ Ｒ Ｎ Ａ Ｒ Ｔ Ｐ Ｃ Ｒ 扩增法检测癌细胞株和胃癌组织端粒酶活性和ｈ Ｔ Ｒ Ｔ Ｒ Ｎ Ａ 表达。结果：８ 株癌细胞和 ６ 例胃癌组织均有端粒酶活性和 ｈ Ｔ Ｒ Ｔ Ｒ Ｎ Ａ 表达；９ 例正常组织、１ 例早期胃癌胃体粘膜和 ５例胃炎标本均无端粒酶活性和ｈ Ｔ Ｒ Ｔ 表达。结论：端粒酶ｈ Ｔ Ｒ Ｔ ｍ Ｒ Ｎ Ａ 的 Ｒ Ｔ Ｐ Ｃ Ｒ 检测方法同端粒酶活性测定 Ｔ Ｒ Ａ Ｐ法有良好的一致性，有可能成为利用端粒酶标志检测癌症的一种更为有效的检测方法。
Objective: To establish a sensitive and fast reverse transcription polymerase chain reaction(RT PCR)technique for human telomerase reverse transcriptase RNA(hTRT RNA) detection, and to investigate the relationship of hTRT RNA and telomerase activity to the clinico pathological properties of gastric cancer. Methods: Telomerase activity and hTRT RNA in malignant cell lines and gastric cancer were analyzed with the use of TRAP and hTRT RNA RT PCR, respectively. Results: Eight malignant cell lines and 6 cases of gastric cancer were positive for telomerase activity and hTRT RNA. Nine normal gastric tissue samples and 1 gastric mucosa sample of early stage tumor and 5 chronic gastritis tissue samples were negative. Conclusion: RT PCR of hTRT RNA is a sensitive, specific and fast method for telomerase detection. The method may be useful in cancer diagnosis as well as TRAP.
出处
《军事医学科学院院刊》
CSCD
北大核心
1999年第3期190-192,196,共4页
Bulletin of the Academy of Military Medical Sciences
基金
国家"八六三"计划(102080401)
博士后科学基金
关键词
端粒酶
端粒酶催化亚基
胃癌
RT-PCR
telomerase
hTRT
gastric tumor
reverse transcription polymerase chain reaction
