摘要
根据鲉形目鱼类Cytb和COⅡ上下游保守序列各自设计引物,对日本鬼鲉的线粒体DNA进行PCR扩增,克隆并测定了Cytb和COⅡ因的全序列,COⅡ基因为691bp,Cytb为1141bp。从GenBank下载28种鲉形目及外群鱼类的Cytb部分或全序列和10种鲉形目及外群鱼类的COⅡ部分或全序列,然后进行比对分析,用NJ法和ML法构建分子系统发育树。系统发育树结果显示:线粒体Cytb和COⅡ基因序列构建的分子系统树,与传统分类方法的结果基本一致;毒鲉科的日本鬼与科的关系最近;在飞角鱼科(Dactylopteridae)是否属于形目还是单独成豹鲂鲉目的问题上,本研究倾向支持飞角鱼科独立归成豹鲂鲉目(Dactylopteriformes)。日本鬼鲉的COⅡ与昆虫类的不同,形目的COⅡ基因比Cytb基因保守,即COⅡ基因比Cytb基因进化慢。COⅡ基因进化树相应的节点上数值稍高。
The entire mitochondrial Cyt b and CO Ⅱ sequence of Inimicus japonicus, 69 1 bp for CO Ⅱand 1141bp for Cyt b, were cloned, whose primers was designed by the upstream and downstream sequences of the mitochondrial Cyt b and CO Ⅱ conserved sequences in Scorpaeniformes. They were compared, respectively, with the mitochondrial Cyt b sequences of 28 other fishes in Scorpaeniformes and the mitochondrial CO II sequences of 10 other fishes in Scorpaeniformes, based on the molecular phylogenetic trees built by the NJ and ML method. Phylogenetie tree showed that： phylogenetie trees constructed by mitochondrial Cyt b and COⅡ sequences were consistent with traditional classification methods; the relations of L japonicus （Synanceiidae） and Scorpaenidae were mostly close; and Dactylopteridae to build up Daetylopteriformes was more reasonable than to be Scorpaenoidei in this paper. Different from the mitochondrial COⅡ in the insects, the mitochondrial COⅡ gene was more conservative than the mitochondrial Cyt b gene in Scorpaeniformes. Therefore, the value of the nodes in the phylogenetic tree of the mitochondrial COⅡ gene was corresponding slightly higher.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2011年第1期47-54,共8页
Oceanologia Et Limnologia Sinica
基金
长江学者和创新团队发展计划项目,IRT0734号
