摘要
马铃薯Y病毒是烟草上的主要病毒之一。目前中国关于PVY分子变异的报道还较少,但变异株系的出现应该引起科研工作者的高度重视。笔者简述PVY的株系分化进程,并从检测技术的优缺点及应用等方面总结PVY检测的指示植物法、酶联免疫法、聚合酶链式反应、核酸杂交等几种方法。指出随着植物种质资源的引进和生态条件的改变,PVY变异株系将不断出现,这对株系检测方法提出了更高的要求,因此在实际应用中要综合运用各种检测方法提高检测的准确性。
Potato virus Y (PVY) has become one of main viruses on tobacco. Now there are few reports on the variation of PVY, but the occurrence of PVY variation should arouse scientific researchers ' attention. This paper introduced the course of strains differentiation of PVY and gave a general account of methods of detecting PVY. With the introduction of seed resources and changes of environment, variation strains of PVY have constantly appeared which put forward a higher requirement to the detection methods, so various detection methods should be used comprehensively to improve the detection accuracy in practice.
出处
《中国农学通报》
CSCD
北大核心
2011年第5期40-43,共4页
Chinese Agricultural Science Bulletin
基金
湖南省烟草专卖局标准项目"烟草马铃薯Y病毒的防控技术研究"(09-11Aa29)
关键词
马铃薯Y病毒
株系分化
检测
potato virus Y
strains differentiation
detection