摘要
对７２ 株临床分离的绿脓杆菌进行了药敏试验， 其中４２ 株检测了β－内酰胺酶及几种β－内酰胺酶基因同源序列在总ＤＮＡ和质粒中的情况，并探讨了绿脓杆菌总ＤＮＡ中不同β－内酰胺酶基因同源序列与不同β－内酰胺类抗生素耐药性的关系。结果显示， 绿脓杆菌有普遍耐药性和多重耐药性， 在４２ 株绿脓杆菌中有３４ 株产β－内酰胺酶， 阳性率８０．９５％ ， β－内酰胺酶基因同源序列在总ＤＮＡ中的检出亦有普遍性和多重性的特点， ４２ 株菌总ＤＮＡ 中检出ＰＳＥ－１、ＴＥＭ－１、ＳＨＶ－２、ＯＸＡ－１、ＯＸＡ－２β－内酰胺酶基因同源序列２～４种不等， 其中有１７ 株菌分离到质粒（１７／４２）， 这１７株菌的质粒均检出１～２ 种β－内酰胺酶基因同源序列不等。从酶基因的类型看，染色体介导的以ＰＳＥ－１型为主，质粒介导的以ＴＥＭ－１ 型为主。Ｌｏｇｉｓｔｉｃ回归分析显示β－内酰胺类抗生素耐药性与β－内酰胺酶基因的关系有３ 种情况： （１） 与一种特定的β－内酰胺酶基因有关； （２） 与几种β－内酰胺酶基因有关； （３） 与β－内酰胺酶基因无关。
Seventy two strains of Pseudomonas Aeruginosa were isolated clinically from nosocomial infection cases in Tongji Hospital. Antimicrobial susceptibility test was carried out for every isolation The β lase and β lactamase genes of total DNA and plasmids in 42 isolates of P. aeruginosa were detected. The relationship between the different β lactamases gene and resistance of P. aeruginosa to different antimicrobial agents was studied. The results showed that P. Aeruginosa possessed the characteristic of general and multiple drug resistance. The β lase was found out by Nitrocefin in 34 strains out of 42 isolates with the rate of positive reaction being 80 95%. The existence of the β lactamase genes also had widespread and multiple characteristics. Two to 4 β lactamase genes of the PSE 1, TEM 1, SHV 1, OXA 1 and OXA 2 type in total DNA of 42 P. Aeruginosa were demonstrated. Plasmids were detected in 17 strains of P. aeruginosa (17/42). These strains harbored 1 to 2 β lactamase genes in the plasmid. Southern analysis showed that the PSE 1 type was dominant in the β lactamase genes of chromosome mediated. The majority of genes are the TEM 1 type in the β lactamase genes of plasmid mediated. The logistic analysis displayed the resistance of P. aeruginosa to different β lactam agents might depend on β lactamase differently: (1) It is affected by the spectial type of β lactamase gene. (2) It is influenced jointly by the many β lactamse genes. (3)It is not attacked by the β lactamase gene.
出处
《同济医科大学学报》
CSCD
1999年第4期340-342,共3页
Acta Universitatis Medicinae Tongji
基金
国家自然科学基金
关键词
绿脓杆菌
耐药性
内酰胺酶基因
抗生素
pseudomonas aeruginosa
\ antimicrobial resistance
\ β lactamase gene
\ logistic analysis
