摘要
采用生物发光技术和酶组织化学方法检测了７５例不育症男子精子中的ＡＴＰ和琥珀酸脱氢酶（ＳＤＨ）。其中精液参数正常者２５ 例， 弱精子症５０ 例。结果发现， 精液参数组精子中ＡＴＰ含量为 （１３６．７±７２．８） ｐｍ ｏｌ／百万个精子（１０６）， 弱精子症组（６９．５±２１．３） ｐｍ ｏｌ／百万个精子（１０６）， 两组有显著性差异（Ｐ＜ ０．０１）。ＳＤＨ主要分布在精子尾部中段， 两组有极显著性差异 （Ｐ＜ ０．０１）。ＳＤＨ 活力与精子活率呈正相关， 研究表明ＳＤＨ 活性减低， 可影响ＡＴＰ的生成，
The levels of ATP and succinate dehydroge nase (SDH) in spermatozoa of 75 infertile men were detected by bioluminecence and enzyme histochemistry Among them 25 cases (group A) had normal parameter of seminal samples, 50(group B) diagnosed as having asthenospermia The experimental results showed that the levels of ATP were (136 7±27 8) mmol/10 6 spermatozoa in group A, (69 5±21 3)mmol/10 6 spermatozoa in group B( P <0 01) SDH was mainly located in the midpiece of sperm flagellum with the differences being significant between the two groups( P <0 01) A positive correlation was observed between SDH activity and sperm motility It was suggested that deficiency of SDH activity may influence production of ATP and shortage of ATP in sperm with asthenospermia may lower sperm motility
出处
《同济医科大学学报》
CSCD
1999年第4期289-291,共3页
Acta Universitatis Medicinae Tongji
基金
湖北省科委自然科学基金
