摘要
对双歧杆菌细胞表面疏水性（ Ｓｕｒｆａｃｅ ｈｙｄｒｏｐｈｏｂｉｌｉｔｙ，简称 Ｓ Ｈ）、 Ｃａ Ｃ Ｏ２ 细胞的粘附性、刺激 Ｒａｗ ２６４７ 细胞及 Ｒａｗ ２６４７ 与 Ｅ Ｌ４ 细胞混合培养细胞产生 Ｔ Ｎ Ｆα的情况等方面进行了研究。发现双歧杆菌对 Ｃａ Ｃ Ｏ２ 细胞的粘附力与其刺激 Ｒａｗ ２６４７ 细胞产生 Ｔ Ｎ Ｆα的能力密切相关，但菌种的疏水性与其粘附细胞的能力却无多大关系。双歧杆菌 Ｂ Ｇ Ｎ４ 如作用于 Ｒａｗ ２６４７ 和 Ｅ Ｌ４ 的混合培养细胞， Ｔ Ｎ Ｆα的产生随着 Ｅ Ｌ４ 细胞浓度的增加而下降。双歧杆菌的疏水性与糖源有关，以葡萄糖为糖源，疏水能力最强，以蔗糖为糖源，疏水能力最差。双歧杆菌的疏水性与生长温度有密切的关系。
Various strains of Bifidobacterium were examined for surface hydrophobicity(SH),adhesion of CaCO 2 cells and ability to modulate TNF α production from murine macrophage cell line,Raw264 7,and T lymphocyte cell line,EL 4.Close Relalionship was found between their adhesion of CaCO 2 cells and ability to modulate TNF α production by RAW264.7 cells.TNF α production stimulated by BGN 4 decreased as the T cell concentration increased when examined using co incubation system of the RAW264.7 and EL 4 cells.The SH(surface hydrophobicity)of Bifidobacterium decreased as the growth temperature increased from 30℃ to 44℃.Glucose grown cells showed higher SH than sucrose grown cells.Further studies on the in vitro macrophage activation,SH and adherence to CaCO 2 cells of Bifidobacterium strains may reveal some implications on their in vivo probiotic effects.
出处
《山东科学》
CAS
1999年第3期29-32,共4页
Shandong Science
