摘要
目的分析北京市西城区2009-2010年流感监测结果。方法由两家哨点医院每周随机采集流感样病例的咽拭子共40～60份,提取病毒RNA,应用real-time PCR法和MDCK细胞分离法进行检测。结果共采集2 077件流感样样本,其中790件real-time PCR检测阳性,总阳性率为38.04%。其中男性样本1 050件,阳性402件(阳性率38.28%),女性样本1 027件,阳性388件(阳性率37.78%)。哨点医院A送检样本1 071件,阳性410件(阳性率38.28%),哨点医院B送检样本1 006件,阳性380件(阳性率37.77%)。阳性率最低的是2009年6月,为1.65%,阳性率最高的是2010年1月,为91.41%,2009年10月至2010年2月期间阳性率在50.00%上下。其中H3N2流感阳性117件,甲型H1N1流感阳性292件,B型流感阳性373件。790件样本经MDCK细胞培养后,共有143件阳性,阳性率为6.88%。结论北京市西城区2009-2010年流感流行高峰为2009年10月～2010年2月。存在H3N2、甲型H1N1和B型三种流感流行,以甲型H1N1和B型流感病毒为优势毒株。
Objective To analyze the influenza surveillance data of 2009-2010 in Xicheng district,Beijing.Methods Forty to sixty throat swabs of influenza-like patients were randomly collected from two hospitals weekly.The viral isolation and identification were performed with the methods of MDCK cell culture and real-time PCR assay.Results The method of real-time PCR confirmed 790 positive samples（out of 2077,38%） in the throat swabs of influenza-like patients.There was no significant difference of influenza positive rates between men（38.28%,402/1 050） and women（37.78%,388/1 027）or between the hospital A（38.28%,410/1 071） and hospital B（37.77%,380/1 006）.The lowest（1.65%） and highest（91.4%） positive rates were found on June,2009 and January 2010,respectively.The positive rate of influenza detection maintained around 50% from October,2009 to February,2010.Among 790 positive samples,117,292 and 373 cases were infected with the strains of H3N2（influenza A）,H1N1（influenza A） and influenza B,respectively.143（6.89%） samples were positive for influenza virus isolation with MDCK cells.Conclusion The epidemical peak seasons of influenza in Xicheng District of Beijing were from October,2009 to February,2010.The predominant influenza viral strains were the H1N1 of type A and type B.
出处
《中国预防医学杂志》
CAS
2011年第3期259-262,共4页
Chinese Preventive Medicine
