摘要
目的：建立高效液相色谱法测定血浆中氟哌啶醇浓度的方法。方法：以乙腈甲醇０ ．０２５ｍｏｌ／Ｌ 磷酸二氢钾（４５∶５∶５０ ，ｖ／ｖ） 为流动相，氯氟哌啶醇作内标，紫外波长２４８ｎｍ 处检测。结果：血浆中氟哌啶醇的最低检测浓度为０ ．５ｎｇ／ｍｌ，平均提取回收率为８６ ．６ ％ ±４ ．９ ％ ，线性范围１ ～５０ｎｇ／ｍｌ（ｒ ＝ ０ ．９９９８） ，日内和日间ＲＳＤ 分别小于８ ％ 和９ ％ 。结论：方法灵敏、快速、准确。
OBJECTIVE:A high performance liquid chromatography procedure was developed for the determination of haloperidol in human plasma.METHOD:The mobile phase consisted of acetonitrile methanol 0.025mol/L potassium dihydrogen phosphate(45∶5∶50,v/v) and UV detection wavelength was set at 248nm.RESULTS:The limit of detection for haloperidol was 0.5 ng/ml.The absolute recovery was 86.6%±4.9%.The linearity was from 1 to 50ng/ml(r=0.9998) and the relative standard deviations of within day and between day were less than 8% and 9%,respectively.CONCLUSION:The method is rapid,sensitive and accurate enough to be used for clinical therapeutic drug monitoring.
出处
《中国现代应用药学》
CAS
CSCD
1999年第5期44-45,共2页
Chinese Journal of Modern Applied Pharmacy
关键词
氟哌啶醇
高效液相色谱
血浆浓度
haloperidol,High performance liquid chromatography
