摘要
建立了一种转基因植物及其产品PCR检测引物筛选方法。以转基因大豆Roundup Ready为材料,设计了5对引物,通过荧光定量PCR方法对其扩增产物的Ct值、引物与模板的结合效率、PCR产物的溶解曲线方面进行分析,证明RRS2引物对是转基因大豆Roundup Ready最佳品系特异性检测引物。
A method was established to screen PCR primers for detection of genetically modified plants and derived products.The transgenic soybean Roundup Ready standard reference was taken as experiment material.Every primer could amplify its product specifically from the standard reference after optimization of specificity PCR efficiency and melting curves.The primer RRS2 was proved to be the best primer for specific validation of transgenic soybean Roundup Ready using fluorescent quantitative PCR by comparison of Ct values and melting curves among the five specific Primers.
出处
《热带农业科学》
2010年第11期10-14,共5页
Chinese Journal of Tropical Agriculture
基金
现代农业产业技术体系建设专项资金(No.nycytx-24)
国家948项目(No.2006-G37
2010-S19)
国家基金项目(No.31070330)
福建省自然科学基金项目(No.2009J05050)
关键词
转基因植物
PCR
检测
引物筛选
Genetically Modified Plants
PCR
Detection
Primer Screening
