摘要
【目的】研究利用真菌诱导的方式合成羟基磷灰石(HAP)。【方法】采用含不同浓度Na2HPO4和CaCO3的PDA(Potato Dextrose Agar Medium)液体培养基,研究黑曲霉作用诱导HAP合成,并用透射电镜(TEM)观察诱导形成的矿物晶体形态和结构、用X射线衍射(XRD)法确定矿物种类。【结果】主要研究结果如下:(1)在含有合适浓度的Na2HPO4和CaCO3的PDA液体培养基中,接入黑曲霉可以诱导HAP晶体的合成。(2)黑曲霉对HAP合成的诱导作用跟反应时间有关,反应时间越长,越有利于生成HAP。分析认为黑曲霉对HAP诱导作用是因其代谢产酸造成对CaCO3的溶解以及菌丝体对Ca2+的富集作用,在菌丝球内先形成白磷钙石,然后进一步转化为羟基磷灰石。【结论】黑曲霉在含Na2HPO4和CaCO3的PDA液体培养基中能诱导羟基磷灰石的生成。由于黑曲霉诱导合成HAP的反应条件温和,制备工艺简单,成本低,因此具有潜在应用前景。
[Objective]The research objective is to induce hydroxyapatite（HAP） synthesis by using fungus.[Methods] We used the PDA（Potato Dextrose Agar Medium） liquid medium containing different concentrations of Na2HPO4 and CaCO3 to study the way Aspergillus niger synthesize HAP,to observe the induced mineral crystal structure and to analyze the induced mineral type with transmission electron microscopy（TEM） and X-ray diffraction（XRD）.[Results]The main results are as follows：（1） A.niger can induce HAP synthesis in PDA liquid medium with the proper concentration of Na2HPO4 and CaCO3.（2） The reaction of A.niger inducing HAP synthesis depends on the time of the response system.Longer time is more advantageous in producing HAP.The main reason for A.niger inducing HAP crystals formation are as follows： fungal metabolism produces the acidic substances to dissolve CaCO3 and the growth mycelia absorbing Ca2 ＋ lead to Ca2 ＋ enriched on the surface,to promote the production of secondary mineral apatite and further transform into HAP in the mycelia spheres.[Conclusion]A.niger can inducing HAP crystals formation in PDA liquid medium containing Na2HPO4 and CaCO3.Considering the importance of HAP in bio-medicine materials and its costly prices,our method for HAP bio-induction is of the temperate response condition,simple preparation craft,and lower cost,which has the potential application prospect.
出处
《微生物学报》
CAS
CSCD
北大核心
2011年第3期417-422,共6页
Acta Microbiologica Sinica
基金
国家自然科学基金委创优群体项目(40721002)
中国科学院百人计划项目~~
关键词
黑曲霉
羟基磷灰石
诱导作用
生物成矿
Aspergillus niger
hydroxyapatite
Induction
Biological mineralization
