摘要
本研究探讨骨髓增生异常综合征(myelodysplastic syndrome,MDS)患者CCAAT/增强子结合蛋白α(CCAAT/enhancer binding protein alpha,C/EBPα)基因的表达及其在MDS发病、病情进展中的意义。应用Taqman探针的实时荧光定量PCR(Real-time quantitative PCR,RQ-PCR)方法检测33例MDS患者和14例正常对照者的骨髓单个核细胞中的c/ebpα mRNA的表达,分析其在MDS中的表达水平变化。结果表明,MDS低危组和高危组c/ebpα mRNA的表达水平均显著低于对照组(分别为t=3.310,p<0.01,t=6.260,p<0.001),且c/ebpαmRNA在MDS高危组中的表达水平较低危组降低(t=2.709,p<0.05)。MDS患者中c/ebpα mRNA表达水平与患者性别、年龄及血象无明显相关性,但MDS患者c/ebpαmRNA低表达组的骨髓中原始细胞比例明显高于c/ebpα mRNA高表达组(p<0.01)。结论:c/ebpα基因表达的下调与MDS的发病密切相关,c/ebpα基因可能是诱导MDS发病的重要分子生物学标志;c/ebpα表达下调的程度与MDS的病情进展有密切关系。
This study was aimed to investigate the expression of CCAAT/enhancer binding protein alpha gene（c/ebpα） in patients with myelodysplastic syndromes（MDS） and to explore the significance of c/ebpα in pathogenesis and progression of MDS.Real time quantitative reverse transcriptase polymerase chain reaction（RQ-PCR）method was used to detect the expression level of c/ebpα mRNA in bone marrow mononuclear cells（BMMNC） of 33 patients with MDS and 14 normal controls.The results showed that the expression level of c/ebpα mRNA in low-risk and high-risk MDS was significantly lower than that of normal controls（p0.01,p0.001,respectively）,moreover,high-risk MDS showed lower c/ebpα mRNA expression compared with low-risk MDS（p0.05）.c/ebpα mRNA expression level in MDS was not correlated with sex,age and peripheral blood cell amount,while the ratio of blast cells in bone marrow was in the c/ebpα mRNA low expression group significantly higher than that in the high expression group（p0.01）.It is concluded that down-regulation of c/ebpα mRNA expression level has closely associated with the pathogenesis of MDS,the c/ebpα may be an important molecular biological marker of MDS;the degree of down-regulated c/ebpα has closely related to the progression of MDS.
出处
《中国实验血液学杂志》
CAS
CSCD
2011年第1期90-93,共4页
Journal of Experimental Hematology
基金
安徽省自然科学基金项目(编号070413257X)
安徽省卫生厅临床应用项目(编号2008B018)
