摘要
本研究旨在探讨红细胞生成素受体(EPOR)在急性白血病(AL)细胞上的表达及其临床意义。收集我院门诊及住院40例急性白血病患者骨髓,并选择24例骨髓正常患者的骨髓作为对照。应用逆转录聚合酶链反应(RT-PCR)方法检测白血病细胞EPOR mRNA的表达。结果显示,急性白血病细胞有EPOR的表达,表达率为57.5%,平均表达量(灰度值)为0.3549±0.2800,均低于正常对照(p<0.05)。急性髓系白血病(AML)与急性淋巴系白血病(ALL)表达率差别无统计学意义(p>0.05),表达量灰度值AML高于ALL(p<0.05)。结论:急性白血病细胞表达EPOR,表达率和平均表达量均低于正常对照(p<0.05)。AML与ALL表达率无显著差异(p>0.05),表达量AML高于ALL(p<0.05)。
This study was aimed to explore the expression of erythropoietin receptor（EPOR） on acute leukemia cells and its clinical significance.Bone marrow of 40 patients with acute leukemia（AL） and 24 patients with normal bone marrow as control group were collected.Samples came from outpatients and inpatients in our hospital.EPOR mRNA was detected by reverse transcription-PCR.The results showed that there was EPOR expression on AL cells,the expression rate was 57.5%,and the average expression level（Gray value） was 0.3549±0.2800,but both were lower than that in control group（p0.05）.There was no significant statistic difference of expression rate between acute myelogenous leukemia（AML） and acute lymphoblastic leukemia（ALL）（p0.05）,and expression level of AML EPOR was higher than that of ALL（p0.05）.It is concluded that there is EPOR expression on AL cells,while the expression rate and expression level are lower than those in control group（p0.05）.There is no significant statistic difference of the expression rate between AML and ALL（p0.05）,and the expression level of AML EPOR is higher than that of ALL（p0.05）.
出处
《中国实验血液学杂志》
CAS
CSCD
2011年第1期15-18,共4页
Journal of Experimental Hematology
