摘要
利用已糖激酶（Ｈｅｘｏｋｉｎａｓｅ 简称ＨＫ）、６磷酸葡萄糖脱氢酶（Ｇｌｕｃｏｓｅ６ｐｈｏｓｐｈａｔｅ ｄｅｈｙｄｒｏｇｅｎａｓｅ 简称（Ｇ６ＰＤＨ）） 偶联而催化葡萄糖反应的原理，通过测定还原型烟酰胺腺嘌呤二核苷酸磷酸（Ｎｉｃｏｔｉｎａｍ ｉｄｅ ａｄｅｎｉｎｅ ｄｉｎｕｃｌｅｏｔｉｄｅ ｐｈｏｓｐｈａｔｅ ｒｅｄｕｃｅｄ ｆｏｒｍ 简称ＮＡＤＰＨ）的吸光度变化率得出其酶促反应速度，采用标准曲线法测定了葡萄糖的含量。检出限为７×１０－ ７ｍ ｏｌ／Ｌ。
Coupled enzymes of hexokinase and glucose 6 phosphate dehydrogenase were used to catalyze the reaction of glucose with 5′ ATP The rate of change in the absorbance of NADPH was determined to obtain the velocity of the enzymatic reaction and the standard curve for that obtained was used to determine glucose in the samples.The detection limit is 7×10 -7 mol/L.The experimental results indicate that the method has advantages of simplicity,sensitivity and accuracy
出处
《分析试验室》
CAS
CSCD
1999年第5期34-36,共3页
Chinese Journal of Analysis Laboratory
基金
国家民委自然科学基金
